6-羧基壳聚糖的制备及血液相容性研究  

Preparation and Blood Compatibility of 6-Carboxy Chitosan

作　　者：杨越冬[1] 于九皋[1] 周永国[2] 啜惠民[2] 王东妮[3] 

机构地区：[1]天津大学理学院化学系,天津300072 [2]河北科技师范学院化学系,秦皇岛066004 [3]青岛市第三人民医院检验科,青岛266041

出　　处：《中国生物医学工程学报》2007年第4期605-609,共5页Chinese Journal of Biomedical Engineering

基　　金：河北省自然科学基金(B2004000402);河北省教育厅科学基金(2004429)。

摘　　要：将壳聚糖溶于0.5mol/L的乙酸,再以NO2选择性氧化壳聚糖分子中氨基葡萄糖单元的6位羟甲基成羧基,制备了6-羧基壳聚糖。红外光谱分析表明该反应选择性较高,产物保持了壳聚糖基本的链结构;扫描电镜和DSC分析发现,6-羧基壳聚糖与壳聚糖的微纤结构不同,其分子构象卷曲,热稳定性较壳聚糖差。抗凝血测试、溶血测试及扫描电镜观察表明:与壳聚糖相比,6-羧基壳聚糖抗凝血性能大大提高,溶血率明显降低,6-羧基壳聚糖具有很好的血液相容性,其血液相容性随壳聚糖氧化度的提高而提高。The hydroxymethyl groups of 2-amino-2-deoxy-D-glucose units in chitosan were selectively oxidized to carboxyl groups with NO2 gas to form 6-carboxy-chitosan in aqueous 0.5mol/L acetic acid and confirmed by FTIR spectra. SEM study and DSC analysis showed that it is spherulite in 6-carboxy-chitosan rather than micro-fiber structure as in chitosan. Antithrombosis test, hemolysis test, and blood cell morphology observation with SEM revealed that 6-carboxy chitosan had superior blood compatibility to chitosan. The blood compatibility of 6-carboxy chitosan was improved with the degree of oxidization increased.

关 键 词：壳聚糖 6-羧基壳聚糖 血液相容性 二氧化氮 

分 类 号：O636[理学—高分子化学]