人宫颈癌基因蛋白B细胞表位及其HLA限制性细胞毒性T细胞表位预测分析  

Prediction of B cell epitopes and HLA restricted CTL epitopes of human cervical cancer oncogene protein

作　　者：刘安定[1] 杨燕[1] 李方和[1] 陆蒙吉[2] 龚非力[3] 杨东亮[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院实验医学研究中心,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院病原生物学系,湖北武汉430030 [3]华中科技大学同济医学院附属同济医院免疫学系,湖北武汉430030

出　　处：《第四军医大学学报》2007年第14期1260-1263,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30571646);国家重大基础研究项目(973)(2005CB522901)

摘　　要：目的:预测人宫颈癌基因(human cervical cancer oncogene,HCCR)蛋白的二级结构,B细胞表位及其HLA-A,B限制性细胞毒性T细胞表位.方法:综合分析二级结构、亲水性、柔韧性、表面可及性与抗原性指数,预测HCCR蛋白的B细胞抗原表位;利用BIMAS,SYFPEITHI和NetCTL方法预测分析其HLA-A*0201限制性CTL表位,运用NetCTL方法对HLA-A的其他等位基因和HLA-B限制性CTL表位进行预测分析.结果:HCCR蛋白的二级结构主要由α-螺旋结构组成,B细胞优势表位位于N端第41～53,216～228,310～325和355～360区段;预测得到5个HLA-A*0201限制性CTL优势表位分别为YLVFLLMYL(152～160),YLFPRQLLI(159～167),LLLHNVVLL(343～351),CLFLGIISI(138～146)和SIPPFA-NYL(145～153),HCCR蛋白HLA-A,B限制CTL表位主要位于胞外区.结论:应用多参数预测HCCR蛋白B细胞表位及其HLA-A,B限制性细胞毒性T细胞表位,为进一步实验鉴定其表位进而制备单克隆抗体和基于HCCR抗原的肿瘤免疫学治疗奠定了基础.AIM： To predict the secondary structure, the B cell epitopes and the HLA-A, B restricted T cell epitopes of human cervical cancer oncogene （ HCCR ） protein. METHODS ： The secondary structure was predicted by the methods of Proteus and SOPMA. The hydrophilicity, surface probability, flexibility and antigenic index were predicted by the methods of Kyte-Doolittle, Emini, Karplus-Schultz and Jameson-wolf, respectively. According to the above methods, the B cell epitopes for HCCR protein were predicted. HLA-A ＊ 0201-restricted T cell epitopes were predicted by BIMAS, SYFPEITHI and NetCTL. And the restricted T cell epitopes of HLA-B and the other alleles of HLA-A were predicted by NetCTL. RESULTS： The secondary structure of HCCR1 protein was mainly composed of α- helix. The B cell epitopes were probably located at or adjacent to the N-terminal No. 41 - 53,216 - 228,310 - 325 and 355 - 360 regions. And the five predominant HLA-A ＊ 0201-restricted T cell epitopes were YLVFLL.MYL（152-160）, YLFPRQLLI（159-153）, LLLHNVVLL（343-351）, CLFLGIISI（138-146） and SIPPFANYL（145-153）. The HLA-A, B restricted T cell epitopes of HCCR protein were probably located at extracelluar domain of protein. CONCLUSION： Prediction of the epitopes of HCCR protein can provide a basis for production of the monoclonal antibody and development of some promising antigen peptides for tumor vaccines.

关 键 词：宫颈肿瘤 癌基因蛋白质类 表住 B淋巴细胞 CFL表位 

分 类 号：R392.1[医药卫生—免疫学]