急性脊髓挫伤性模型的制备及观察  

Establishment and observation of acute contusive spinal cord injury model

作　　者：祖波[1] 尹宗生[1] 张辉[1] 

机构地区：[1]安徽医科大学第一附属医院骨科,安徽合肥230022

出　　处：《第四军医大学学报》2007年第14期1264-1267,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目(30571881);安徽省自然科学基金资助项目(050430713)

摘　　要：目的:制备急性挫伤型脊髓损伤动物模型.通过观察其行为学变化和脊髓组织的病理改变,为临床治疗脊髓损伤提供理论依据.方法:成年雌性SD大鼠,咬除T10棘突及相应椎板,用改良的Allen法制成脊髓背侧挫伤型模型,对照组仅咬除椎板,分不同的时间行为学评分和病理检测.结果:对照组麻醉恢复后能站立行走,脊髓结构正常;实验组行为学评分较低,脊髓损伤区域出现明显的病理改变,但随着时间的推移,可以部分的恢复.结论:实验组和对照组相比具有显著差异,此模型的建立为进一步了解挫伤型脊髓损伤的发病机制,研究此损伤的病理生理改变及筛选有效的预防治疗提供了良好的基础.AIM： To observe the functional consequence and pathological changes of spinal cord tissues in acute contusive spinal cord injury model so as to provide theoretical evidence for the clinical therapy of the impaired spinal cord. METHODS： In female adult Sprague Dawley rats, after the T10 spinous process and the corresponding vertebral lamina were removed, moderate spinal cord contusive injuries were produced by using modified Allen＇s method in the experiment group. In control group, rats received only T10 laminectomies . The behavioral scores and the pathological alterations were recorded at different time points. RESULTS： In control group, the rats could walk after reviving, and the micro-structure of spinal cord was normal. The behavioral scores of the expreriment group were lower as compared with those of the control group at different time points. The pathological alterations were very apparent in the damaged spinal cord area. When time went on, the spinal cord could be in partial functional recovery. The experimental group showed marked differences from control group. CONCLUSION： The model established in this article will contribute to understanding pathophysiological changes and screening effective therapeutic measures for this injury.

关 键 词：脊髓损伤 疾病模型 动物 大鼠 病理学 

分 类 号：R651.2[医药卫生—外科学]