钙网蛋白在不同疼痛模型大鼠脊髓中的表达  

Calreticulin expression in spinal cord of rat models of different pains

作　　者：王金韬[1] 罗爱林[1] 金小高[1] 张广雄[1] 罗辉宇[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室,湖北武汉430030

出　　处：《第四军医大学学报》2007年第14期1268-1271,共4页Journal of the Fourth Military Medical University

摘　　要：目的:观察钙网蛋白在三种疼痛模型大鼠的脊髓中表达的变化,探讨其与疼痛的可能关系.方法:分别制作甲醛炎性痛模型、保留性坐骨神经分支损伤(SNI)模型和慢性缩窄性坐骨神经损伤(CCI)模型,观察大鼠疼痛行为学改变,并分别用免疫组化和Western Blot技术检测大鼠脊髓c-Fos和钙网蛋白的表达.结果:与对照组相比,各模型组大鼠脊髓后角c-Fos阳性神经元增多(P<0.05);SNI模型组和CCI模型组大鼠脊髓钙网蛋白的表达均上调(P<0.05),甲醛炎性模型组大鼠脊髓钙网蛋白变化无统计学意义(P>0.05).结论:脊髓钙网蛋白的表达上调与SNI和CCI模型的慢性神经病理痛发生过程有关.AIM： To detect the change of rat spinal calreticulin expression levels in three pain models and to investigate the possible involvement of calreticulin in pain development. METHODS： Formalin inflammatory pain model, spared never injury（SNI） model, and chronic constriction injury（CI） model were established. Then, the behavioral changes were observed and evaluated and c-Fos and calreticulin expressions in the spinal cord of rats were detected with immunohistochemistry and Western Blot, respectively. RESULTS： Compared with control groups, quantity of c-Fos positive neurons significantly increased in spinal dorsal horn of rats with formalin injection, SNI or CCI （ P 〈 0.05）; calreticulin expression levels were up-regulated significantly in spinal cord of rats with SNI and CCI （ P 〈 0.05 ）, but not in spinal cord of rats with formalin injection （ P 〉 0.05 ）. CONCLUSION： Up-regulation of calreticulin expression in spinal cord is involved in the developmeut of chronic neuropathic pain induced by SNI and CCI.

关 键 词：疼痛 脊髓 钙网蛋白 

分 类 号：R614[医药卫生—麻醉学]