Temperature Influences on the Expression of GFP Promoted by the Upstream Sequence of cpcB from Arthrospira platensis  

作　　者：LU Yongzhong ZHANG Xuecheng 

机构地区：[1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China [2]Qingdao University of Science and Technology, Qingdao 266042, P. R. China

出　　处：《Journal of Ocean University of China》2007年第3期244-248,共5页中国海洋大学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （No. 30471317）.

摘　　要：In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene(gfp) as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence,suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation ini-tiation region(TIR) revealed that RNA thermosensor might account for the temperature regulation.In order to investigate the regulation mechanism of the phycocyanin gene, a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene （gfp） as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence, suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation initiation region （TIR） revealed that RNA thermosensor might account for the temperature regulation.

关 键 词：CYANOBACTERIUM flow cytometry mutation PHYCOCYANIN PROMOTER RNA thermosensor 

分 类 号：Q178.53[生物学—水生生物学]