人精子甘露糖受体对地鼠卵母细胞超微结构的影响  

Ultrastructural Effects of Mannose Receptor of Human Sperm on Hamster Oocytes

作　　者：江澍[1] 林典梁[1] 陈文列[2] 江一平[1] 

机构地区：[1]福建医科大学细胞与发育工程研究中心 [2]福建中医学院中西医结合研究院,福州350108

出　　处：《福建医科大学学报》2007年第4期293-295,共3页Journal of Fujian Medical University

基　　金：国家自然科学基金资助项目(30271371);福建省自然科学基金资助项目(Z0516042);福建省教育厅科技资助项目(JA03124)

摘　　要：目的研究人精子甘露糖受体(MR)在精卵融合中的作用。方法去透明带金黄地鼠卵经不同浓度的纯化人精子MR作用后,用透射电子显微镜观察卵母细胞超微结构的变化。结果MR作用卵母细胞后,卵母细胞内的皮质颗粒(CG)减少,并伴随有CG胞吐现象,同时卵母细胞表面的微绒毛数量减少。结论MR作用于卵母细胞表面后,可触发CG胞吐,并促使卵母细胞表面微绒毛减少,提示MR在介导精子与卵母细胞识别与融合中起作用。Objective To study the intermedisting role of mannose receptor（MR） of human spermatozoa in sperm-egg recognition and fusion. Methods The zona free hamster eggs were pre-incubated with MR which isolated from motile sperm of human by mannose-agarose gel affinity chromatography. The ultrastructural alternation of hamster eggs were examined by transmission electron microscope. Results The incubation of MR decreased cortical granules（CG） in the oocytes with the CG exocytosis and the quantity of the microvillus on the oolemma reduced with dosage dependent manner. Conclusion The MR induced the CG exocytosis and impel the microvillus reduction. These changes indicated that MR of human sperm may play an intermediate role in sperm-egg recognition and fusion.

关 键 词：甘露糖 精子 精子卵子相互作用 膜融合 卵母细胞 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]