波兰小麦和矮兰麦45S rDNA和5S rDNA基因位点FISH分析  

FISH analysis of 45S rDNA and 5S rDNA genes in Triticum polonicum L.and T.turgidum L.cv.Ailanmai

作　　者：廖进秋[1] 杨瑞武[1] 周永红[2] 辻本壽[3] 

机构地区：[1]四川农业大学生命科学与理学院,雅安625014 [2]四川农业大学小麦研究所,都江堰611830 [3]鸟取大学农学部植物遗传育种研究室

出　　处：《遗传》2007年第4期449-454,共6页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30270099);教育部长江学者和创新团队发展计划(编号:IRT0453)资助~~

摘　　要：采用双色荧光原位杂交技术,以45S rDNA和5S rDNA基因为探针,对波兰小麦(Triticum polonicum L.)和矮兰麦(T.turgidum L.cv.Ailanmai)进行了分析。结果表明,高秆波兰小麦(T.polonicum L.High)和矮兰麦的45SrDNA和5SrDNA基因位点高度一致,都显示4个45S rDNA和6个5S rDNA基因位点;矮秆波兰小麦(T.polonicumL.Dwarf)的45S rDNA基因位点与高秆波兰小麦和矮兰麦也一致表现出4个位点,而其5S rDNA基因位点有8个。同时讨论了rDNA基因位点的数目和分布位置在种间和种内存在差异的原因。Using the method of double color fluorescence in situ hybridization （FISH）, we had analyzed Triticum polonicum L. and Z turgidum L. cv. Ailanmai with the probes of 45S rDNA and 5S rDNA. The results indicated that there were highly consistent in T. polonicum L. High and T. turgidum L. cv. Ailanmai, both having four 45S rDNA loci and six 5S rDNA loci. In Z polonicum L. Dwarf, there were also four 45S rDNA loci, the same as that in T. polonicum L. High and T. turgidum L. cv. Ailanmai, but there were eight 5S rDNA loci. At the same time, we discussed the reason of interspecific and intraspecific variation of the two types of rDNA in locus number and location between T. polonicum L. and T. turgidum L. cv. Ailanmai.

关 键 词：波兰小麦 矮兰麦 RDNA基因 荧光原位杂交 

分 类 号：S512.1[农业科学—作物学]