先天性自身免疫性感音神经性聋防治的实验研究  

Experimental study on prevention and treatment of congenital autoimmune sensorineural hearing loss in guinea pigs

作　　者：董伟达 谭长强 周红[2] 陆玲[1] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院耳鼻咽喉科,耳鼻咽喉科学研究室,江苏南京210029 [2]南京医科大学基础医学院生理中心,江苏南京210029

出　　处：《中国耳鼻咽喉头颈外科》2007年第7期417-420,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(30471876);江苏省自然科学基金资助项目(BK-2003058)联合资助

摘　　要：目的探讨免疫抑制剂环磷酰胺是否可以有效地预防和治疗先天性自身免疫性感音神经性聋。方法同种粗制内耳抗原免疫雌性豚鼠,在妊娠期间给予免疫抑制剂环磷酰胺预防,对于未给予免疫抑制的母鼠所产子鼠给予环磷酰胺治疗,观察实验动物内耳听觉功能和病理形态学变化。结果同时给予免疫抑制的母鼠及其各自所产子鼠均未出现明显的听觉损伤和内耳病理改变;出现听觉功能障碍和内耳病理变化的子鼠经环磷酰胺治疗后,其听觉功能有所提高(主要为低频区)。结论环磷酰胺可有效地预防母体内针对内耳组织特异性自身免疫反应所造成的子鼠先天性感音神经性聋,但疗效有限。To study whether immunosuppressant （IS, cyclophosphamide, CPA） have the function of prevention and treatment of congenitalautoimmune sensorineural hearing loss or not. METHODS The female guinea pigs were immunized by crude conspecific inner ear antigens （CIEAgs）, and took orally IS or not at the same time during gestation. Then the pregnant guinea pig＇s and their offspring＇s hearing function were measured and inner ear histopathologic changes were observed. Some of offspring borne by the female guinea pigs which immunized with CIEAgs and not treated with IS showed hearing loss and were treated by IS, and their hearing functions were measured and inner ear histopathologic changes were observed with same techniques. RESULTS After immunizing with CIEAgs, some of female guinea pigs which immunized with CIEAgs and not treated with IS showed different degrees of hearing loss （the thresholds of acoustic nerve compound active potential and cochlear microphonic potentials elevated） and immune inflammations in inner ear tissues. All females guinea pigs in experimental group which immunized with CIEAgs and treated with IS at same time, and their offspring have no any hearing loss and inner ear histopathologic changes. After IS therapy, the hearing function improved （mainly at the low- frequency region） in some offspring guinea pigs, which borne by the female guinea pigs which immunized with CIEAgs and not treated with IS. CONCLUSION IS could effectively prevent offspring＇s congenital sensorineural hearing loss induced by their mother＇s special antibodies against inner ear tissues antigens. IS showed effective result for treatment of congenital autoimune sensorineural hearing loss, but the curative effect was limited.

关 键 词：豚鼠 动物 实验 聋 迷路 

分 类 号：R764[医药卫生—耳鼻咽喉科]