异烟肼处理前后耻垢分枝杆菌mc^2 155细胞蛋白双向电泳图谱的差异分析  

Comparison of Proteins Expression of Mycobacterium Smegmatis mc^2 155 after Treatments with Isoniazid (INH) by Two-dimensional Electrophoresis

作　　者：湛垚垚[1] 张翠丽[2] 辛毅[2] 

机构地区：[1]大连医科大学生物化学与分子生物学教研室,辽宁大连116027 [2]大连医科大学生物技术专业,辽宁大连116027

出　　处：《临床军医杂志》2007年第4期485-488,共4页Clinical Journal of Medical Officers

基　　金：国家自然科学基金(30570416)资助

摘　　要：目的利用蛋白质组学方法,识别异烟肼处理前后耻垢分枝杆菌mc2 155细胞(SM细胞)后差异表达的蛋白质,为进一步深入探讨异烟肼抑制分枝杆菌生长的作用机制及寻找新的抗结核药物作用靶标奠定基础。方法观察不同浓度的异烟肼处理前后耻垢分枝杆菌生长曲线,确定药物处理SM细胞的最佳时间和浓度,并提取在最佳处理时间和浓度条件下的SM细胞总蛋白,采用载体两性电解质pH梯度双向凝胶电泳技术分离异烟肼处理前后耻垢分枝杆菌mc2 155细胞的总蛋白质,用PDuest7.3.1图像分析软件比较分析,以识别差异表达的蛋白质。结果在空白对照组检出159个蛋白斑点,在异烟肼处理组检出221个蛋白斑点,在相对分子质量30 000~90 000和pH值4.5~6.0范围内,异烟肼处理组蛋白斑点数均多于空白对照组。结论异烟肼处理前后耻垢分枝杆菌mc2155细胞的蛋白质组具有差异,提示异烟肼处理后促进了某些特定基因的表达。Objective To investigate the microbiostatic mechanism of isoniazid （INH） so as to find out differential expression proteins in mycobactcrium smcgmatis mc^2155 cells treated with isoniazid. Methods The total proteins of untreated and INH- treated mc^2155 cells were separated by ampholyte pH gradient-based two-dimensional gel electrophoresis （2-DE）. The differential expression proteins were analyzed by using image analysis software. Results Proteins of control group and INH-treated group contained 159 and 221 protein spots, respectively. Within both relative molecular weight range （30.0 - 90.0 kDa） and pH value （ 4.5 - 6.0） , INH-treated group＇ s protein spots were more than control group＇ s. Conclusion There is a significant difference in protein level between untreated and INH-treated mc^2155 cells. It suggests that INH can increase the expression of certain genes in mc^2155 cells.

关 键 词：异烟肼 耻垢分枝杆菌mc^2 155 双向电泳 蛋白质组比较 

分 类 号：R378.91[医药卫生—病原生物学]