ELISA(双抗体夹心法)共系统检测抗-HBe与HBeAg结果分析  

AnaLysis on the Detection of SampLe Anti-HBe and HBeAg by ELISA in a Common Measurement System with HBeAg EIA Reagent Kits of a DoubLe-Antibody Sandwich Method

作　　者：李宏杰[1] 方磊[1] 程家坤[2] 祖华[1] 

机构地区：[1]安徽省淮南新华医疗集团新华医院临床检验中心 [2]安徽省淮南新华医疗集团新华医院核医学科,安徽淮南232052

出　　处：《中国现代医生》2007年第08S期14-16,28,共4页China Modern Doctor

摘　　要：目的探讨用双抗体夹心法检测HBeAgEIA商品试剂盒共系统检测抗-HBe与HBeAg的可行性及结果判定方法。方法用双抗体夹心法检测HBeAgELISA试剂盒结合配套中和(竞争)抑制法检测抗-HBeELISA试剂盒中的中和试剂(HBeAg)等试剂组分共系统检测1000份随机血清(浆)标本抗-HBe与HBeAg,并分别以常规ELISA检测抗-HBe与HBeAg作平行对照。结果ELISA共系统检测抗-HBe与HBeAg易于目测;它们的比色判定临界值同常规ELISA易于设定(或使用临界值血清,检测HBeAg时也使用抗-HBe阴性对照),与常规ELISA检测比色判定无显著性差异(配对计数资料比较的χ2检验:相关检验均为P<0.005,υ=1,a=0.05;抗-HBe优劣检验(ELISA共系统检测抗-HBe前两项COV与抗-HBe临界值血清)均为P>0.900,υ=1,a=0.05;HBeAg优劣检验依次为HBeAg临界值血清:(1)P>0.900,υ=1,a=0.05,抗-HBe阴性对照;(2)0.100>P>0.050,υ=1,a=0.05,ELISA共系统检测HBeAgCOV;(3)0.100>P>0.050,υ=1,a=0.05,但χ21<χ22<χ23)。结论用双抗体夹心法检测HBeAgELISA试剂盒与配套中和(竞争)抑制法检测抗-HBeELISA试剂盒试剂组分重组共系统检测抗-HBe与HBeAg是可行的,两种试剂盒能够相互替代,在有优质现代酶标仪的实验室,可以省去常规ELISA试剂盒其中之一,实用价廉,值得推广。Objective To investigate the practicality and how to deal with the results of detecting sample Anti-HBe and HBeAg by ELISA in a common measurement system with HBeAg EIA （coating purified HBeAb） reagent kits of a doubLe-antibody sandwich method. Methods Both Anti-HBe and HBeAg of 1000 random serum（or pLasma） samples were measured by ELISA in a common measurement system with HBeAg ELISA reagent kits of a double-antibody sandwich method and a nuetralizing reagent（HBeAg） and other reagent components in Anti-HBe EIA reagent kits of a nuetralizing and competitive inhibition method and the results were aslo respectively detected by a routine ELISA as a control. ResuLts The results of detecting sample Anti-HBe and HBeAg in the common measurement system are easy to be differentiated with one＇s eyes;their cut off value（COV） is similar to a routine ELISA and easier to be set up （or serum of COV was used, Anti-HBe negative control was too used while detecting HBeAg） and the quanlitative assay of OD measurements was no significant defference between ELISA in a common measurement system and a routine ELISA （ x^ 2test of enumeration data with paried design： correlation test all are P〈0.005, v = 1, a=0.05; Anti-HBe difference test（the fwst two COV of Anti-HBe EIA in a common measurement system and Anti-HBe serum of COV ）all are P 〉0.900, v =1, a-0.05;HBeAg difference test is in proper order HBeAg serum of COV（1） P〉0.900, v =1, a=0.05, Anti-HBe negative control; （2） 0.100〉P 〉0.050, v =1, a=0.05, COV of HBeAg EIA in a common measurement system; （3） 0.100 〉P〉0.050, v =1, a=0.05, however x^21 〈 x ^22 〈 x^2 3）. ConcLusion The detection of sample Anti-HBe and HBeAg by ELISA in a common measurement system with HBeAg ELISA reagent kits of a doubLe-antibedy sandwich method and Anti-HBe EIA reagent kits of a nuetraLizing and competitive inhibition method to match combining their reagent components is practicable,this two reagent kits are abLe to replace each other,if a modern a

关 键 词：酶联免疫吸附试验 双抗体夹心法 中和(竞争)抑制法 共系统检测 临界值判定标准 

分 类 号：R446.62[医药卫生—诊断学]