宫颈扩张过程中单核细胞趋化蛋白-1表达的意义  

Expression and effect of monocyte chemotactic protein-1 during delivery

作　　者：段彼得[1] 刘锡梅[1] 王立杰[1] 耿岩[1] 张建梅[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012

出　　处：《现代妇产科进展》2007年第6期443-445,共3页Progress in Obstetrics and Gynecology

摘　　要：目的：观察单核细胞趋化蛋白-1（MCP-1）在分娩期宫颈扩张不同程度时母血、子宫肌层组织和胎盘组织中的表达,探讨MCP-1在宫颈扩张过程中的作用。方法：选择临产后急诊行剖宫产终止妊娠的初产妇42例,根据术前30min宫颈扩张程度分为A组10例（宫颈口开大〈2cm）,B组10例（宫颈口开大2~4cm）,C组10例（宫颈口开大4~6cm）,D组12例（宫颈口开大〉6cm）。酶联免疫吸附法测定产妇血清中MCP-1水平;用半定量RT-PCR法测定子宫下段肌层及胎盘组织中MCP-1mRNA的表达。结果：C组和D组血清MCP-1水平和组织中MCP-1mRNA含量显著高于A组和B组（P〈0.01）,B组组织中MCP-1mRNA含量显著高于A组（P〈0.05）;4组产妇MCP-1的表达量与宫颈扩张程度呈正相关。结论：MCP-1在宫颈扩张过程中表达增加,在宫颈扩张过程中起重要作用。Objective： To determine the expression of monocyte chemotactic protein-1 （MCP-1） in the blood,lower uterine segment and placenta during parturition at term and to investigate its correlation with the stage of cervical dilatation. Methods：Biopsy specimens were taken from the lower uterine segment of 42 women undergoing cesarean section at term. The stage of cervical dilatation 30 minutes before the time of cesarean section was 〈 2cm （ group A,n = 10）, 2 to 〈 4 cm （ group B,n = 10） ,4 to 6 cm （ group C ,n = 10） or 〉 6cm （ group D,n = 12）. Enzyme linked immunosorbent assay （ ELISA ） was used to measure concentrations of MCP-1 in the blood. The expression of MCP-1 mRNA in lower segment of uterus and placenta from the group of cesarean section were studied by semi-quantitative RT-PCR. Results ：The concentration of MCP-1 in serum and the expression of MCP-1 mRNA in tissue were group D than group A and group B （P 〈 0.01 ）. The group B sue （P 〈 0.05 ）, the expression of MCP-1 was correlated with vical dilatation is associated with up-regulation of MCP-1 in significantly greater at group C and was also higher than group A in tiscervical dilatation. Conclusion： Cervical dilatation is associated with up-regulation of MCP-1 in the lower uterine segment. MCP-1 is a key mediator in maturation and dilatation of uterine cervix.

关 键 词：宫颈成熟 子宫肌层 胎盘 单核细胞趋化蛋白1 

分 类 号：R714.1[医药卫生—妇产科学]