扶正解毒汤对硫酸镍诱导16HBE细胞微核形成的抑制作用  

Inhibitory Effect of Fuzheng Jiedu Decoction on Micronuclei Formation Induced by Nickel Sulfate in 16HBE Cells

作　　者：吕晓云[1] 蒲晓丽[1] 朱玉真[1] 白德成[2] 王敏[1] 

机构地区：[1]兰州大学中西医结合研究所,兰州730000 [2]兰州大学医学实验中心,兰州730000

出　　处：《癌变．畸变．突变》2007年第4期309-312,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：教育部博士点专项科研基金(20050730031)

摘　　要：背景与目的:研究中药扶正解毒汤(FJD)对硫酸镍(NiSO4)诱导人支气管上皮细胞(16HBE)微核形成的抑制作用。材料与方法:分别以NiSO4暴露、不同浓度FJD含药血清与NiSO4共同处理体外培养的16HBE细胞,观察其微核率的变化,并采用激光扫描共聚焦显微镜检测不同处理组细胞内Ni2+、Mg2+、Zn2+浓度及自由基含量的变化。结果:与阴性对照组相比,NiSO4(400μmol/L)暴露组细胞微核率、细胞内Ni2+浓度及自由基含量均明显增高(P<0.01),而细胞内Mg2+、Zn2+浓度则明显降低(P<0.01)。与NiSO4暴露组相比,低、中、高剂量FJD血清与NiSO4共处理组细胞微核率分别为(62.4±11.5)‰、(35.2±7.1)‰及(27.6±5.6)‰,显著低于NiSO4暴露组(P<0.05,P<0.01),细胞内Ni2+浓度及自由基含量下降,Mg2+、Zn2+浓度升高(P<0.05,P<0.01)。结论:FJD对镍诱导16HBE细胞微核形成有明显的抑制作用,其机制可能与调节染镍细胞内某些金属元素含量变化及清除自由基有关。BACKGROUND ＆ AIM：To study the inhibitory effect of Fuzheng Jiedu Decoction（FJD） on the micronuclei formation induced by nickel sulfate （NiSO4） in human bronchial epithelial cell line（16HBE）. MATERIALS AND METHODS： Cultured 16HBE cells were exposed to NiSO4（400μmol/L） in vitro. Different doses of FJD drug serum were added into the media containing NiSO4.Micronuclei（MN） were scored in 1 000 16HBE cells.Cellular ions of Ni^2＋, Mg^2＋ and Zn^2＋ and free radicals were all measured with laser scanning confocal microscopy（LSCM）. RESULTS： After exposure of 16HBE cells to NiSO4（400 μmol/L）,MN frequencies, Ni^2＋ and free radicals increased and Mg^2＋,Zn^2＋ decreased,compared with that of negative control（P〈0.01） Compared with NiSO4 exposure group（10.8%）, the MN frequencies in groups treated with both FJD and NiSO4 decreased significantly （P 〈 0.05, P 〈 0.01）. In cells co-treated with FJD and NiSO4, Ni^2＋ and free radicals decreased and Mg^2＋, Zn^2＋ increased （P〈0.05, P〈0.01） . CONCLUSION： By restoring the alteration of some metal ions and scavenging free radicals, FJD could suppress micronuclei formation in 16HBE cells induced by nickel.

关 键 词：扶正解毒汤 含药血清 硫酸镍 微核 离子 自由基 

分 类 号：R285.5[医药卫生—中药学]