In situ AFM observation of BSA adsorption on HOPG with nanobubble  

作　　者：WU ZhiHua ZHANG XueHua ZHANG XiaoDong SUN JieLin DONG YaMing HU Jun 

机构地区：[1]Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China [2]Nanobiology Laboratory, College of Life Science, Shanghai Jiao Tong University, Shanghai 200030, China [3]Department of Chemical and Biomolecular Engineering, University of Melbourne, Vic. 3010, Australia [4]Department of Chemistry, Life and Environment Science School, Shanghai Normal University, Shanghai 200234, China [5]Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

出　　处：《Chinese Science Bulletin》2007年第14期1913-1919,共7页

基　　金：National Natural Science Foundation of China (Grant No. 20403010)

摘　　要：Nanobubbles have been proven existent at the liquid/solid interface, and become a focus of research on varied interfacial processes. In the present work, by observing in situ with atomic force microscope (AFM), we found that nanobubbles could influence the adsorption process of bovine serum albumin (BSA) on hydrophobic surface of highly ordered pyrolytic graphite (HOPG). BSA could adsorb evenly, and coexist with nanobubbles at water/HOPG interface. After removing nanobubbles by injecting ethanol, some hollows were found in the BSA layers at the same positions of nanobubbles existing previously. These hollows were about 8 nm in depth and dozens of nanometers in diameter. The correlation coefficient between the areas of nanobubbles and that of the corresponding hollows reached 0.88―0.94, which strongly supported the assumption that the hollows were indeed caused by the nanobubbles. Moreover, the BSA molecules formed rings around the nanobubbles, suggesting the preference of BSA adsorption onto the contact line at water/HOPG interface.Nanobubbles have been proven existent at the liquid/solid interface, and become a focus of research on varied interfacial processes. In the present work, by observing in situ with atomic force microscope （AFM）, we found that nanobubbles could influence the adsorption process of bovine serum albumin （BSA） on hydrophobic surface of highly ordered pyrolytic graphite （HOPG）. BSA could adsorb evenly, and coexist with nanobubbles at water/HOPG interface. After removing nanobubbles by injecting ethanol, some hollows were found in the BSA layers at the same positions of nanobubbles existing previously. These hollows were about 8 nm in depth and dozens of nanometers in diameter. The correlation coefficient between the areas of nanobubbles and that of the corresponding hollows reached 0.88-0.94, which strongly supported the assumption that the hollows were indeed caused by the nanobubbles. Moreover, the BSA molecules formed rings around the nanobubbles, suggesting the preference of BSA adsorption onto the contact line at water/HOPG interface.

关 键 词：牛血清白蛋白 热解石墨 显微镜 原子吸附 

分 类 号：Q51[生物学—生物化学]