乙烯利调控甘蔗生长前期基因表达初报  

Differential Gene Expression in Sugarcane Leaves Regulated by Ethephon during Early Growth Stage

作　　者：魏源文[1] 胡春锦[2] 邓智年[1] 李杨瑞[1] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室 [2]广西农业科学院植物保护研究所,南宁530007

出　　处：《热带亚热带植物学报》2007年第4期290-293,共4页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(39860039);广西科技能力创新与条件建设项目(桂科能0630006-5F)资助

摘　　要：在甘蔗(Saccharum of ficinarum L.)生长前期分别叶面喷施200mg L-1乙烯利和清水,利用cDNA-AFLP技术分析甘蔗经不同处理后体内基因表达的差异情况,以期通过对差异表达基因进行序列分析和功能分析,探讨乙烯利调控甘蔗生长的分子机理。结果表明:经乙烯利处理后的甘蔗叶片,cDNA-AFLP的扩增产物多态性丰富,基因表达差异显著;部分差异片断序列与抗病、抗逆及促进光合作用等相关基因具有较高的同源性。A study was conducted to investigate differential gene expression in sugarcane （Saccharum officinarum L. cv. ROC16） leaves subjected to 200 mg L^-1 ethephon, cDNA-AFLP amplification showed a high level of polymorphism, and gene expression was significantly different compared with the control. Some sequences of different expression fragments were highly homologous to disease-resistant and photosynthesis-associated genes.

关 键 词：甘蔗 乙烯利 CDNA-AFLP 基因差异表达 

分 类 号：S566.1[农业科学—作物学]