花烛无菌苗液体增殖培养的影响因子  

Auxiliary Bud Proliferation of Anthurium andraeanum Lind. in Liquid Culture

作　　者：徐彬[1] 陈海钟[1] 郭维明[1] 王广东[1] 文方德 金剑平 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]珠海市园艺研究所,广东珠海519070

出　　处：《热带亚热带植物学报》2007年第4期338-342,共5页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金(30300244);广东省科技厅项目(K130532002-1-4);南京农业大学SRT项目(0303A08)资助

摘　　要：以花烛(Anthurium andraeanum Lind.)品种Sonate无菌苗为材料,研究了液体培养条件下不同植物生长调节剂、蔗糖浓度、培养方式等因子对腋芽增殖的影响,并比较了Sonate、Valentino和Julanba 3个品种间腋芽增殖的差异。结果表明:在液体振荡培养条件下,以Nitsch+BA0.5mgL-1+KT1.0mgL-1+蔗糖30gL-1为Sonate品种适宜的增殖培养基;在20μmolm-2s-1弱光下培养25d后转至40μmolm-2s-1正常光照下培养,对Sonate品种的增殖效果较好,腋芽诱导数平均可达11.1个,腋芽平均长度为1.4cm。花烛腋芽增殖能力在品种间存在差异,但3个品种均可在以上培养基和光照条件下快速增殖,其中以Sonate和Valentino品种的增殖较快。The influences of different combinations of plant growth regulators, sucrose concentration and culture modes on proliferation rates and the quality of auxiliary buds were studied in Anthurium andraeanum cv. Sonate in liquid culture. Differences in the proliferation were compared among A. andraeanum cvs. Sonate, Valentino and Julanba. Nitsch basal medium supplemented with 0.5 mg L^-1 BA, 1.0 mg L^-1 KT and 30 g L^-1 sucrose was suitable for auxiliary bud proliferation of Sonate under liquid flask-shaking culture condition. Auxiliary bud proliferation of Sonate was promoted by the light of 20 μmol m^-2s^-1 of 25-day culture followed by 40 μmol m^-2s^-1, with the bud proliferation rate of 11.1 and the average bud length of 1.4 cm. The cultivars, Sonate, Valentino and Julanba, were well propagated under the above culture condition, especially Sonate and Valentino, but they showed differences in auxiliary bud emergence.

关 键 词：花烛 液体培养 腋芽增殖 

分 类 号：S682.14[农业科学—观赏园艺]