CD151对脐静脉内皮细胞Akt-Ser^(473)位点磷酸化和细胞增殖的影响  

The effect of CD151 on Akt-Ser^(473) phosphorylation and human umbilical veine endothelial cell proliferation

作　　者：郑振中[1] 刘正湘[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心血管内科,湖北武汉430030

出　　处：《医学研究生学报》2007年第3期229-232,I0001,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30570728);湖北省卫生厅科研基金重点资助项目(批准号:JX2A04)

摘　　要：目的:研究CD151对脐静脉内皮细胞(HUVEC)丝/苏氨酸蛋白激酶(Akt)、磷酸化Akt-Ser473(p-Akt-Ser473)表达和细胞增殖的影响。方法:通过构建pAAV-CD151质粒并转染HUVEC,HUVEC分为正常对照组、绿色荧光蛋白(GFP)组和CD151组。Western Blot检测CD151及Akt、p-Akt-Ser473表达,MTT法测定脐静脉内皮细胞的增殖能力。结果:①CD151组CD151及p-Akt-Ser473表达明显增加,CD151组与正常对照组和GFP组比较有显著性差异(P<0.05),而总Akt三组间无明显差异(P>0.05)。②MTT法检测正常对照组、pAAV-GFP转染组、pAAV-CD151转染组的A值分别为0.1663±0.0095、0.169±0.0085和0.2457±0.0044,pAAV-CD151转染组较pAAV-GFP转染组和正常对照组细胞增殖能力增强,且有显著性差异(P<0.05)。结论:CD151具有促进内皮细胞增殖的作用。CD151可能是通过激活p-Akt-Ser473,增加Akt活力,达到促进细胞增殖的作用。Objective：To investigate the the effect of CD151 on Akt-Ser^473 and human umbilical veine endothelial cell proliferation. Methods：To construct pAAV-CD151 and transiently transfect to HUVEC （ human umbilical veine endothelical） mediated with Lipofectamine 2000. HUVEC were assigned to control group, GFP group, CD151 group. After transfection,the expression of CD151 ,Akt and phospho-Akt were measured by western blot. Proliferation of HUVEC were measured by MTF colorimetric assay. Results ：（1）The expression of CD151, phospho-Akt of pAAV-CD151 transfected group were significantly increased compared with non-transfected group and pAAV-GFP transfected group （ P 〈 0.05 ）.（2） Proliferation of HUVEC of pAAV-CD151 transfected group was higher than these non-transfected and pAAV- GFP transfected group （ P 〈 0.05 ）, results of which were 0.166 3 ± 0.009 5,0.169 ± 0. 008 5,0. 245 7 ± 0.0044 in MTT assay. Conclusion：We found that CD151 may phosphorylate Akt at Ser^473 and increase Akt acivity,thus induce cell proliferation. Akt is involved in CD151-induced cell proliferation.

关 键 词：CD151 丝/苏氨酸蛋白激酶 磷酸化 脐静脉内皮细胞 细胞增殖 

分 类 号：Q782[生物学—分子生物学]