双探针实时荧光定量法检测HCV RNA抗原的临床意义  

Clinical significance of detection of HCV RNA by using double probed real-time fluorescence quantitiative determinatior

作　　者：徐邦牢[1] 吴瑜霞[1] 王蓉[1] 陈英姿[1] 雷秀霞[1] 

机构地区：[1]广州市第一人民医院,广东广州510180

出　　处：《中国热带医学》2007年第8期1287-1288,共2页China Tropical Medicine

基　　金：广州市医药卫生科技项目(2005-YB-024);广东省医学科研基金项目(A2006518);广州市科技攻关计划项目(2004Z3-E0441)

摘　　要：目的建立双探针荧光定量HCV RNA检测方法,分析其定量检测HCV RNA的灵敏度和特异性以及HCV RNA含量与患者病情的相关性。方法选取100例抗HCV抗体阳性样本,包括慢性肝炎患者45例、肝硬化患者30例、肝癌患者25例,并选取献血员50例为对照,用双探针荧光定量法和两种商品荧光定量试剂方法进行HCV RNA检测。结果双探针荧光定量法阳性率为93%(93例);两种商品荧光定量试剂方法阳性率分别为84%(84例)和79%(79例)。结论双探针荧光定量提高HCV RNA检测的灵敏度和特异性,HCV RNA含量与丙型肝炎患者病情变化及严重程度相关。Objective To establish real- time fluorescence quantitative reverse transcription polymerase chain reaction （RFQ- RT - PCR） for determianfion of HCV RNA and evaluatate the significance of RFQ - RT - PCR in determnianton of HCV RNA by using double - probes. Methods A total of 100 anti - HCV positive cases and 50 anti - HCV negative cases were determined with RFQ - RT- PCR by using double probe, and were also detected by other two HCV RNA quantification kits simultaneously. Results HCV RNA positive rate was 93% （93 cases） in RFQ- RT- PCR by using double- probes,while it was 84% （84 cases） and 79% （79cases） by using other two kits. Conclusion RFQ-RT- PCR assay using doubleprobes can enhance the sensifivity and specificity of HCV RNA detection. HCV RNA load may be associated with the progress and severity of disease.

关 键 词：HCV RNA 双探针 荧光定量 丙型肝炎 

分 类 号：R373.2[医药卫生—病原生物学]