超声波诱导人肝癌多药耐药细胞株HepG2/ADM凋亡的实验研究  

Experimental study on the apoptosis of human liver carcinoma MDR cells induced by low-frequence pulse ultrasound exposure

作　　者：翟宝进[1] 郭玉棉[1] 王志宏[2] 伍烽[3] 齐秋俊[4] 

机构地区：[1]武警医学院附属医院脑系科,天津300162 [2]武警医学院附属医院科研科,天津300162 [3]重庆医科大学医学超声工程研究所,重庆400016 [4]天津市河东区中医院,天津300162

出　　处：《武警医学院学报》2007年第4期341-343,347,F0002,共5页Acta Academiae Medicinae CPAPF

基　　金：国家自然科学基金资助项目(30200060)

摘　　要：【目的】探讨治疗超声对人肝癌多药耐药细胞株HepG2/ADM凋亡的影响及其可能机制。【方法】研究对象分为4组,分别为对照组、阿霉素组、超声波组(10 min)、阿霉素+超声波组。以频率为800 KHz,声强为3.0 W/cm2,时间10 min的脉冲式超声波作用于细胞。荧光显微镜观察细胞形态变化;DNA片段化分析检测染色体断裂情况;原位末端标记(TUNEL)和流式细胞仪检测细胞凋亡。【结果】经超声波作用10 min后,HepG2/ADM细胞死亡具有细胞凋亡的形态学特征;阿霉素组和超声波组(10 min)细胞凋亡的比例明显高于对照组;阿霉素+超声波联合治疗显著增加细胞凋亡率,为30.1%。【结论】治疗超声照射10 min可诱导HepG2/ADM人肝癌细胞株凋亡,联合阿霉素治疗,可显著增加细胞凋亡比例。[Objective] To explore the effect of low-frequence pulse ultrasound （US） on human hepatoma MDR cells and mechanism. [Methods] HepG2/ADM cells were cultured in vitro and randomly divided into 4 groups： control group （HepG2/ADM only）, group ADM （adding 0.5ug/ml adriamycin for 1 h）, group US （low-frequence pulse ultrasound for 10 min）, and group US combinated with adriamycin simultaneously. A pulsed US sonication at a frequency of 800 KHz was delivered with an intensity level of 3.0 W/cm^2 . The apoptosis of HepG2/ADM was evaluated by fluorescence microscopy, DNA fragmentation assay and flow cytometry assay. [Results] HepG2/ADM cells radiated by US had the typical characteristics of apoptosis. Comparaed with the control group （HepG2/ADM）, the apoptosis rates were higher in ADM or/and US group. The therapeutic alliance of US and ADM for MDR ceils, have a significant change in the ratio of apoptosis （30.1%）. [Conclusions] The US sonication of 800KHz could induce apoptosis of HpG2/ADM cell in vitro , and could act synergistically with adriamycin.

关 键 词：超声波 肿瘤 多药耐药 凋亡 

分 类 号：Q255[生物学—细胞生物学]