快眼动睡眠剥夺对抑郁模型大鼠不同脑区色氨酸羟化酶和单胺氧化酶A的影响  

Effects of REM sleep deprivation on behavioral depression and brain Tryptophan hydroxylase and Monoamine Oxidase in rats treated with chronic stresses.

作　　者：朱列和[1] 许崇涛[2] 

机构地区：[1]惠州市中心人民医院心理门诊,广东惠州516001 [2]汕头大学精神卫生中心,广东汕头515063

出　　处：《中国卫生产业》2007年第07X期14-15,17,共3页China Health Industry

基　　金：广东省自然科学基金资助项目（04020238）

摘　　要：目的 探讨睡眠剥夺对抑郁模型大鼠不同脑区色氨酸羟化酶（TPH）和单胺氧化酶A（MAO-A）的影响。方法 对抑郁模型大鼠进行睡眠剥夺，应用旷场实验观察大鼠实验前后自主行为的变化，用紫外分光光度计和免疫印迹技术检测大鼠大脑中脑、海马、下丘脑和前额叶四个部位的MAO-A的活性和TPH。结果 抑郁模型大鼠经过睡眠剥夺后自主行为显著增加，睡眠剥夺后大鼠海马、中脑、下丘脑和额叶的TPH显著升高，大鼠海马MAO-A活性显著增加。结论 睡眠剥夺对抑郁模型大鼠的抑郁样行为有改善作用，可能与色氨酸羟化酶和单胺氧化酶A有关。Objective To explore the effect of Rapid eye movement sleep deprivation （REMSD） on behavioral depression and activities of cerebral tryptophan hydroxylase （TPH） and monoamine oxidase A （ MAO - A） in rats treated with chronic stresses. Methods REMSD was performed by flow- erpot technique. An open field test was repeated three times for evaluating the depressive - behavior in rats during the experiment. Expression of TPH and activities of MAO - A in hippocampus, frontal cortex, hypothalamus and mid - brain were measured by western - blotting and UV - spectropho- tometer respectively. Results Open field tests showed that 72h REMSD reversed the depressive - behavior of rats remarkably. There was significant difference in activities of TPH in hippecampus, mid - brain, hypothalamus and front cortex between chronic stress rats and 72h REMSD rats. Further- more the difference in increased activity of MAO - A in hypothalamus between chronic stress rats and 72h REMSD rats was also significant. Conclusion REMSD may improve the depressive - behavior in rats. It may be correlated to the increased activities of both TPH and MAO - A .

关 键 词：快眼动睡眠剥夺 抑郁症 色氨酸羟化酶 单胺氧化酶A 

分 类 号：R285.5[医药卫生—中药学]