CD45mAb及联合应用供体脾细胞对大鼠移植心脏生存时间的影响  

The effect of vein injecting of CD45mAb or associated with donor splenocytes to heart allografts survival time.

作　　者：李敬来[1] 萧明第[2] 

机构地区：[1]北京大学深圳医院心血管外科,广东深圳518036 [2]上海交通大学医学院附属上海市第一人民医院心血管外科,上海200000

出　　处：《中国卫生产业》2007年第07X期20-21,共2页China Health Industry

摘　　要：目的 观察术中静脉应用CD45mAb及供体脾细胞对移植心脏生存时间的影响。方法 建立大鼠异位心脏移植模型。术中静脉应用CD45mAb及供体脾细胞。结果 用CD45mAb组移植心脏生存时间较未用药组有明显延长（P=0.006〈0．05）。CD45mAb＋供体脾细胞组移植心脏的生存时间与单独应用CD45mAb组没有明显差异（P=0．855〉0．05），但比单独用脾细胞组（P=0．008，〈0．05）和未用药组有明显延长（P=0．009，〈0．05）。结论 术中静脉应用CD45mAb及联合应用供体脾细胞均可延长移植心脏存活时间；单独应用供体脾细胞不能延长移植心脏的生存时间。Objective To observe the heart allografts survival time after administration of immunosuppressive agents CD45mAb or associated with donor splenocytes on the time of operation. Methods A simplified heterotopic rat heart transplantation model was established and the CD45mAb （ 1 mg/kg） were vein injected or asseeiated with donor splenoeytes （ 1 × 10^9 cells/100g）. Results In using CD45mAb group, the average of the survival of heart allograft have a significant difference compared with those of the no therapy group （ P = 0. 006 〈 0.05 ）. In using CD45mAb ＋ donor splenoeytes group, the average survival of heart allagrafts have no significant difference with those of using CD45 mAb alone （ P = 0. 855 〉 0.05 ）, and much longer than those of using donor splenocytes group （ P = 0. 008 〈 0.05 ） and no therapy group （ P = 0. 009 〈 0.05 ）. Coulusion The findings demonstrate that a single injection of CD45mAb in rat can induce stable long - term acceptance of heart allografts. The donor splenocytes vein injection alone can not prolong the survival time of heart allagrafts.

关 键 词：CD45单克隆抗体 脾细胞 心脏移植 

分 类 号：R654.2[医药卫生—外科学]