人乳头瘤病毒16型L1蛋白的纯化及免疫学活性  

Purification and Immunological Activity of L1 Protein of Human Papillomavirus Type 16

作　　者：安静[1] 周旭[1] 席亮[1] 包红[1] 陈汉泉[1] 

机构地区：[1]兰州生物制品研究所第二研究室,兰州730046

出　　处：《中国生物制品学杂志》2007年第7期522-524,共3页Chinese Journal of Biologicals

摘　　要：目的纯化人乳头瘤病毒16型(HPV16)L1蛋白,并分析其免疫学活性,为诊断试剂和疫苗的研制奠定基础。方法采用电洗脱的方法纯化目的蛋白,通过免疫双扩散和ELISA法检测其免疫反应性。将该蛋白免疫BALB/c小鼠,分析其免疫原性。结果纯化后的目的蛋白经凝胶灰度扫描显示,其纯度达到95%,免疫双扩散和ELISA结果显示,宫颈癌病人血清抗体效价明显高于健康人,表明该蛋白具有良好的抗原性,小鼠免疫力试验结果表明该蛋白具有较好的免疫原性。结论电洗脱法得到的目的蛋白纯度较高,且有较好的免疫学活性。Objective To purify the L1 protein of human papillomavirus type 16 （ HPV16 L1 ） , study its immunological activity and lay a foundation of developing diagnostic kit and vaccine. Methods Purify HPV16 L1 protein by electroelution and determine its immunoreactivity by double immunodiffusion test and ELISA. Immunize BALB/c mice with the purified HPV16 L1 protein and determine the serum antibody. Results The purity of HPV16 L1 protein after purification reached 95%. Both double immunodiffusion test and ELISA showed that the serum antibody titers of patients with cervical cancer were significantly higher than those of healthy person, which indicated good antigenicity of HPV16 L1 protein. The protein also showed good immunogenicity in mice. Conclusion The HPV16 L1 protein purified by electroelution was of high purity and good immunological activity.

关 键 词：人乳头瘤病毒 L1蛋白 纯化 免疫学活性 

分 类 号：R373.9[医药卫生—病原生物学]