条斑紫菜多糖的分离纯化与抗肿瘤活性  

Study on Isolation, Purification and Antitumor Activity of Polysaccharides from Porphyra yezoensis

作　　者：顾佳雯[1] 张陆曦[1] 徐红丽[2] 郭婷婷[1] 何培民[1] 

机构地区：[1]上海水产大学生命学院中心实验室,上海200090 [2]第二军医大学生物化学与分子生物学教研室,上海200433

出　　处：《中国生物工程杂志》2007年第7期50-54,共5页China Biotechnology

基　　金：上海市科委重大攻关项目(054319936);上海市科委浦江人才计划项目(05PJ14086);上海市教委优势(重点)学科资助项目(Y1101)

摘　　要：目的:应用生化提纯技术从条斑紫菜(Porphyra yezoensis)中提取不同纯度的多糖,并观察各多糖组分对人乳腺癌细胞MCF-7生长的影响。方法:通过DEAE-52柱层析和Sephadex G-200柱层析,对条斑紫菜粗多糖热水提取物进行纯化;经HPLC,紫外和红外光谱对多糖各组分的纯度及结构进行初步鉴定;并用MTT法和流式细胞仪检测多糖对人乳腺癌细胞MCF-7生长的影响。结果:分离纯化出的各组分条斑紫菜粗多糖对人乳腺癌细胞MCF-7生长均有不同程度的抑制作用,其中PY-D2可诱导MCF-7细胞凋亡。结论:条斑紫菜多糖具有杀伤肿瘤细胞MCF-7的作用。The polysaccharide from Porphyra yezoensis （PYP） was purified and analyzed by biochemical techniques. The effect of PEP on the proliferation of MCF-7 was investigated in vitro. Methods： The purified polysaccharide from Porphyra yezoensis was obtained by DEAE- cellulose and Sephadex G-200 column chromatography. The effect of PEP on the proliferation of MCF-7 was investigated in vitro by MTT method. Two components, PY-D1 and PY-D2, were isolated from the Porphyra yezoensis polysaccharide by ion-exchange chromatography DEAE-52. Two polysaccharides, PY-G1 and PY-G2, were further isolated from PY-D2 Ueda by gel filtration Sephadex G-200. PY-G was identified by UV and IR spectra. All of components could inhibit the proliferation of MCF-7. Results： It was found polysaccharides of Porphyra yezoensis had antitumor activity for MCF-7 cells.

关 键 词：条斑紫菜多糖 分离纯化 MTT法 MCF-7 

分 类 号：Q539[生物学—生物化学]