胃癌及癌旁组织定量比较蛋白质组学研究  

Quantitative and Comparative Proteomics Analysis of Gastric Cancer and Adjacent Noncancerous Tissues

作　　者：罗治文[1] 谢笑娟[1] 马桂兰 呼建文[3] 刘丽杰[1] 葛文松[1] 朱樑[1] 

机构地区：[1]第二军医大学附属长征医院消化科,上海200003 [2]解放军第513医院,兰州732750 [3]中国科学院上海生命科学院蛋白质组研究分析中心,上海200031

出　　处：《中国生物工程杂志》2007年第7期55-60,共6页China Biotechnology

基　　金：上海市科学技术委员会科研计划项目(05ZR14102);上海市科学技术委员会科技攻关重点项目(041409008)

摘　　要：为寻找胃癌特异的肿瘤标记物,用于胃癌临床诊断及药物治疗靶点的选择,采用荧光差异显示凝胶电泳(DIGE)技术分离并筛选Cy3、Cy5及Cy2荧光素标记的胃癌及对应癌旁组织差异表达蛋白质,用基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)或串联质谱技术进行鉴定并分析。结果共筛选出33个差异表达蛋白质点,其中9个蛋白质点在胃癌组织中上调,24个蛋白质点下调。对22个蛋白质点采用质谱技术成功鉴定,突变结蛋白、锰超氧化物歧化酶、热休克蛋白60等在胃癌中高表达,热休克蛋白27、前列腺素F合酶、硒结合蛋白1、锌指蛋白160、微管蛋白α6、真核生物翻译延伸因子1α1等在胃癌组织中低表达,并筛选出5个未知蛋白。这些差异表达蛋白可望成为胃癌诊断的特异标记物,并与胃癌的发生、发展及预后等有关,为胃癌的诊断、发生机制的研究提供了新的思路。To identify specific protein markers for gastric cancer detection and diagnosis, as well as develop new potential therapeutic targets of the disease. Gastric cancer tissues and adjacent normal mucosa were examined by the fluorescence differential in-gel electrophoresis （DIGE） technique after labeled with CyDye DIGE flnors Cy3,Cy5 and Cy2. Protein spots detected differential with statistical significance were identified by MALDI-TOF MS or MS/MS. As the result,intensity changes of 33 spots were detected with statistical significance. 9 protein spots of them up-regulated otherwise 24 down-regulated in gastric cancer tissues. And 22 of them were identified by MALDI-TOF MS or MS/MS successfully. Several proteins up-regulated such as MnSOD, HSP60, mutant desmin et al. HSP27, prostaglandin F synthase,SeBP 1,zinc finger protein 160,tubulin alpha 6,eTEF1 A 1 and so on were down-regulated. The results suggest that DIGE is a useful technique for differential expressed proteins screening and analysis in gastric cancer tissues which may be useful for the development of new molecular markers for diagnosis and prognosis of gastric carcinoma. This differently expressed proteins may be useful tumor markers for gastric cancer.

关 键 词：胃癌 肿瘤标记物 DIGE 定量蛋白质组学 

分 类 号：Q819[生物学—生物工程]