运用均匀设计法优化rPA(K)在原核系统中的表达条件  

Using Uniform Design to Optimize Expressive Conditions of rPA(K) in E.coli

作　　者：罗惠霞[1] 李敏[1] 王玉炯[1] 马春骥[1] 

机构地区：[1]西部特色生物资源保护与利用教育部院重点实验室,银川750021

出　　处：《中国生物工程杂志》2007年第7期94-99,共6页China Biotechnology

基　　金：宁夏自然科学基金资助项目(NZ0505)

摘　　要：运用均匀设计法对影响rPA(K)在大肠杆菌中表达的因素进行了优化设计,摸索出了对表达产物进行诱导表达的最佳优化表达条件。结果如下:溶解氧为90%,IPTG诱导时间为5.3h、IPTG终浓度为0.1mmol/L、培养基为HD、pH值为6.0、诱导温度为25℃。目的蛋白平均密度从0.16提高到0.48,是优化前的3倍。Using uniform design to optimize some relative factors influencing the expressing of t-PA variant- rPA（K） in E. coli system, and found the best expressive conditions. They were（ by using 300ml culture fask） ： the volume of media was 25ml, inducing time was 5.3 hours, pH was 6.0, the concentration of IPTG was 0. 1mmol/L, inducing time was 25℃, and best culture media was HD. After being optimized, the yield of expression had been improved from 0.16 to 0.48, and it was as 3 times as before. The results above will offer the basement for purification and renaturation of rPA （K）.

关 键 词：RPA(K) 均匀设计 优化表达 

分 类 号：Q819[生物学—生物工程]