2型糖尿病大鼠逼尿肌收缩功能改变的实验研究  

An experimental study on changes of contractile function of detrusor muscles of type 2 diabetes mellitus rats

作　　者：高宏飞[1] 双卫兵[1] 高俊平[1] 翟晓梅[1] 王东文[1] 

机构地区：[1]山西医科大学第一医院

出　　处：《护理研究（下旬版）》2007年第7期1899-1900,共2页Chinese Nursing Researsh

基　　金：国家自然基金(30571865);山西省归国留学人员基金(晋留管办发[2005]6号

摘　　要：[目的]研究2型糖尿病膀胱逼尿肌收缩功能的改变和M3受体表达的变化。[方法]建立2型糖尿病大鼠模型,在糖尿病病程第16周,应用离体膀胱灌注方法观察逼尿肌收缩功能的变化;RT-PCR和Western blotting方法观察逼尿肌M3受体mRNA表达的变化。[结果]2型糖尿病组逼尿肌收缩功能低于正常对照组,2型糖尿病两组大鼠膀胱内压为(17.61±3.62)cmH2O/100mg,正常对照组为(24.57±4.20)cmH2O/100mg;2型糖尿病组逼尿肌M3受体mRNA和蛋白的表达均高于正常对照组,分别为63.53%±3.54%和39.37%±3.70%,43.53%±1.59%和25.39%±1.40%。[结论]2型糖尿病大鼠逼尿肌M3受体的生物合成是上调的,但是逼尿肌的收缩功能却降低,可能是M3受体功能方面存在着一定的障碍。Objective： To investigate on the changes of contractile function of detrusor muscles of type 2 diabetes mellitus （DM） rats and expression changes of M3 receptor of detrusor muscles. Methods：Type 2 DM model rats were prepared. Changes of contractile function of detrusor muscles were observed by using isolated irrigation of bladder at the 16th week of DM course. Changes of m - RNA and M3 receptor proteins of rats were observed by adopting RT-PCR and Western blotting method. Results： The contractile function of detrusor muscles in type 2 DM group was worse than that in control group, internal pressure of bladder in type 2 DM group was （17.61 ± 3.62 ） cm H20/100 mg, and （24.57 ± 4.20） cmH2O/100mg in normal control group; expression of m - RNA and M3 receptor protein of detrusor muscles in type 2 DM group was higher than that in normal control group, respectively（63.53 ± 3.54）% and （39.37±3.70）% and （43.53±1.59）% and（25.39± 1.40）%. Conclusion： Biosynthesis of M3 receptors of detrusor muscles of type 2 DM rat is up - regulated. But the contractile function of detrusor muscles decreased. The reasons are supposed possibly that there were some disorders existed in M3 receptor function.

关 键 词：2型糖尿病 膀胱 逼尿肌 M3受体 

分 类 号：R471[医药卫生—护理学]