心叶驼绒藜胚器官培养及再生体系的研究  

Study in Regenerated Plants Induced from Embryo of Ceratoides ewersmanniana

作　　者：郑晓红 李柱 付爱良 杨刚 沙吾列 

机构地区：[1]新疆维吾尔自治区草原研究所,新疆乌鲁木齐830000

出　　处：《中国草地学报》2007年第4期50-55,共6页Chinese Journal of Grassland

基　　金：新疆维吾尔自治区科技攻关和重点科技项目(200231111)

摘　　要：以心叶驼绒藜无菌苗叶片、茎段、幼根为外植体,对通过器官发生的途径发育成再生植株进行了研究。结果表明:(1)、无菌苗的建立:用1/2MS+琼脂+蔗糖,出苗率高(90%以上)、苗壮。(2)、诱导培养基:用MS1+IAA0.2mg/L+KT0.1mg/L培养基,出愈率低,没有获得再生植株;用MS2+IAA0.2mg/L+KT0.2mg/L、MS3+IAA0.1mg/L+KT0.1mg/L培养基,叶片、茎段具有再生植株发育的能力,出愈率较高。(3)、生根培养基:用1/2MS+NAA0.1 mg/L,生根率高、周期短;用1/2MS+IAA0.1 mg/L,生根率低,周期长。By using leaves, stem sections and young roots as explants, establishment the development of regeneration plant from organ was studied, results revealed that. 1. the establishment of germfree plantlet. Used 1/2MS ＋ agar ＋ sucrose, the burgeoning rate was high and plant was strong. 2. induction culture medium. Used culture medium MS1 ＋IAA 0.2mg/L＋KT 0. 1mg/L, callus induction rate was low and there was no regeneration; used MS2＋IAA 0. 2mg/L＋ KT 0.2mg/L, MS3 ＋ IAA 0. 1mg/L ＋ KT 0. 1mg/L, the leaves, stem section obtain the power of regeneration, callus induction rate was high. 3. rooting culture medium. Used 1/2MS ＋ NAA 0. lmg/L, the rooting rate was high and cycle was short/Used 1/2MS ＋ IAA 0. 1mg/L, the rate was low, and cycle was long.

关 键 词：心叶驼绒藜 组织培养 愈伤组织 再生植株 

分 类 号：Q943.1[生物学—植物学]