丹参注射液对重症急性胰腺炎大鼠胰腺NF-κB活化的影响  

Influence of Radix Salviae Miltiorrhizae Injection upon NF-κB in Rat with Severe Acute Pancreatitis

作　　者：郑邦瑞[1] 沈文律[1] 文军[1] 杨少华[1] 陈少逸[1] 

机构地区：[1]汕头大学医学院第二附属医院普外科,汕头515041

出　　处：《中国普外基础与临床杂志》2007年第4期392-395,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的观察重症急性胰腺炎(SAP)大鼠胰腺核因子-κB(NF-κB)活性的变化及丹参注射液对其的影响,从而探讨丹参注射液治疗急性胰腺炎的机理。方法将大鼠随机分为正常对照组、SAP组和丹参治疗组3组,采用腹腔和皮下同时注射L-精氨酸的方法制成大鼠急性胰腺炎模型,分别测量血浆、腹水淀粉酶,并采用免疫组化方法测定胰腺组织中NF-κB的表达。结果与正常对照组比较,SAP组血浆、腹水淀粉酶水平明显增高,胰腺组织NF-κB表达强度升高(P<0.05);丹参治疗组上述指标与SAP组相比均有明显下降(P<0.05),且胰腺的病理损伤减轻,存活时间更长。结论丹参注射液可能通过降低胰腺组织中NF-κB的表达而对急性胰腺炎的治疗发挥作用。Objective To investigate the mechanism of radix salviae miltiorrhizae （RSM） injection in treatment of pancreatitis through observing the changes of activity of nuclear factor-kappa B （NF-κB） in pancreas of rats with severe acute pancreatitis （SAP） and the influence of RSM injection upon NF-κB in pancreas tissue. Methods Seventy-five rats were randomly divided into 3 groups： normal control group, SAP group and RSM treatment group, which were injected with normal saline, normal saline or RSM in the peritoneal cavity, respectively. The model of SAP rats was made by injecting L-arginine into peritoneal cavity and by subcutaneous injection at the same time. The concentrations of amylase in plasma and in ascites were measured respectively, and the expression of NF- κB in pancreas tissues was determined by immunohistochemistry. Results The levels of amylase in plasm and ascites in SAP group and RSM treatment group increased significantly with the increased expression of NF-κB in pancreas tissue, but it was also found that both the level of amylase and the expression of NF-κB in RSM treatment group were significantly lower compared with those in SAP group, and the survival time of RSM treatment group was longer than SAP group with less pathological injury in the pancreas tissues. Conclusion RSM may be effective for the treatment of pancreatitis by degrading the expression of NF-κB.

关 键 词：重症急性胰腺炎 核因子-ΚB 丹参注射液 

分 类 号：R285.5[医药卫生—中药学]