塔玛亚历山大藻藻际细菌溶藻过程  

Lysis of Alexandrium tamarense mediated by bacteria in its phycosphere

作　　者：王新[1] 周立红[1] 郑天凌[1] 宁修仁[2] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]国家海洋局海洋生态系统与生物地球化学重点实验室,杭州310012

出　　处：《生态学报》2007年第7期2864-2871,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(30370276);高等学校博士学科点专项科研基金资助项目(20050384002);国家海洋局海洋生态系统与生物地球化学重点实验室开放基金资助项目(LMEB200601)~~

摘　　要：海洋微藻在生长过程中向周围环境分泌多种胞外产物,形成细菌自由生长的藻际环境,藻际细菌对微藻的生长有一定的调控作用。在指数生长期的塔玛亚历山大藻培养液中加入φ为1%的2216E培养基,在加入2216E后16h内藻细胞全部裂解。用数码显微镜记录了藻细胞形态变化,分别用DAPI法和荧光模拟底物法测定了细菌数量、胞外酶活性变化,结果表明:在溶藻过程中细菌数量、胞外酶活性在第6小时到第10小时增加了50～100倍。塔玛亚历山大藻藻际细菌主要分布在藻细胞表面,其群落结构改变和数量剧增是溶藻的主要原因,细菌分泌的β-葡萄糖苷酶和几丁质酶可能在溶藻过程中起重要作用。Bacteria can freely grow in the phycosphere of marine alga which produce extracellular substances to their surrounding environment. These bacteria could regulate the growth of algae. One percent （v/v） of 2216E medium was mixed into Alexandrium tamarense culture system. These results show that algal cells were all lysed within 16 hours. After adding 2216E medium, both the abundance and the extracellular enzyme activity of bacteria increased by 50- 100 times from the 6^th hour to the 10^th hour in lysis of algae. These experiments suggest that bacteria in the phycosphere could mainly attach to the surface of Alexandrium tamarense. Changes in the population of this community might cause the lysis in algae. The β-D-glucosidase and chitinase produced by bacteria could play a major role in lysis of algae.

关 键 词：塔玛亚历山大藻 藻际环境 胞外酶活性 藻菌关系 赤潮防治 

分 类 号：Q938[生物学—微生物学] Q948.8