慢性应激对大鼠海马Akt/FKHRL1信号通路活性的影响  

Activation of Akt/FKHRL1 Signaling Pathway after Chronic Stress in the Rat Hippocampus

作　　者：朱婉儿[1] 胡长春[1] 谢文婷[1] 吴丽霞[1] 陈芝芸[2] 梅垣宏行[3] 

机构地区：[1]浙江大学医学院医学心理学,杭州310058 [2]浙江省中医院消化病研究室,杭州310006 [3]日本名古屋大学医学部老年科

出　　处：《心理学报》2007年第4期656-661,共6页Acta Psychologica Sinica

基　　金：教育部留学回国人员科研启动基金(J20040252);浙江省自然科学基金(2005Y013);浙江大学2004"挑战杯"学生科研基金资助项目

摘　　要：探讨慢性应激对大鼠海马Akt/FKHRL1信号通路活性的影响,及其应激源特异性。将24只雄性SD大鼠随机分为心理应激组、束缚应激组和正常对照组(n=8)。用Western-blotting方法测定28天应激后海马Akt、FKHRL1蛋白含量及其磷酸化水平。结果表明,应激后三组大鼠海马磷酸化Akt、FKHRL1含量差异有统计学意义(p<0.01;p<0.001),束缚应激组低于正常对照组(p<0.01)。提示慢性应激能导致海马磷酸化Akt、FKHRL1表达水平降低,但其影响程度与应激源特异性有关。Introduction There is now a substantial body of evidence which suggests that chronic stress can increase susceptibility to disease such as depression and posttraumatic stress disorder. Chronic stress induces neuronal atrophy and death in the cortex as well as in the hippocampus. The proto - oncogene protein kinase B （PKB）, also known as Akt, is a central player in a signaling pathway of which many components have been linked to cellular survival. Akt as well as of its downstream targets Forkhead transcription factors （forkhead homologue in rhabdomyosarcoma, FKHR） have emerged in recent years as cardinal pathway underlying cellular survival and opposing apoptosis in neurons. But the activation of Akt/FKHRL signaling pathway after chronic stress in the brain remains poorly defined. We hypothesized that repeated immobilization stress exposure may change the levels of phosphorylation of Akt （pAkt） and FKHRL1 （pFKHRL1） in the rat hippocampus. The study was designed to analyze the effect of chronic stress on activation of Akt/FKHRL1 signaling pathway in the rat hippocampus. Methods Twenty four male Sprague Downey rats （280 ±20）g were purchased from Shanghai Laboratory Animal Center, Chinese Academy Sciences and were used for all experiments. Rats were randomly divided into three groups： psychologically stressed group, immobilization stressed group, and controls. Each group contained eight rats. Animals were housed in groups of 4 under standard laboratory conditions in temperature - controlled rooms （24℃）, and maintained on a 12 h light/dark cycle （lights on at 08.00） with food pellets and water available ad libitum. Rats of the two stressed groups were subjected to immobilization stress or psychological stress one hour per day for 28 consecutive days, respectively. At the end of the experimental period, the animals were decapitated and their hippocampi were rapidly removed and kept at - 80℃ until analysis. The protein level of Akt, FKHRL1 and phosphorylation of Akt, FKHRL1 were det

关 键 词：应激 海马 AKT FKHRL1 磷酸化. 

分 类 号：B845[哲学宗教—心理学]