实验犬留置不同类型气管支架的基础研究  

The basic research on different intra-tracheal stent placement in experiment dogs

作　　者：刘兆玉[1] 郎旭[1] 卢再鸣[1] 张军[1] 温峰[1] 郭启勇[1] 

机构地区：[1]沈阳中国医科大学附属盛京医院介入病房,110001

出　　处：《介入放射学杂志》2007年第7期485-488,共4页Journal of Interventional Radiology

摘　　要：目的探讨不同类型气管覆膜支架植入在实验动物中的应用价值。方法健康成年杂种犬18只,随机平均分为3组,气管内留置不同类型支架,A组留置全覆膜支架(全长6cm覆膜),B组留置中段覆膜支架(全长6cm,上下两端各1cm不覆膜,覆膜长度4cm),C组留置裸支架(全长6cm)。术后观察动物进食、排痰、呼吸情况,饲养20周后分别处死。观察气管标本大体和HE镜下改变,并行增殖细胞核抗原(PCNA)染色,定量分析增生细胞的阳性表达。结果各组实验犬均未发生支架留置后的急性呼吸困难,有轻至中度呛咳,经观察,排痰量并无明显差异。均可正常饮水、进食,发声无异常。部份犬活动减少。支架不覆膜处,气管内壁组织增生较明显,气管腔狭窄程度大于支架覆膜处。各组HE染色镜下均表现为炎性反应,低柱状上皮细胞鳞状化生、肉芽增生和纤维增生性改变。3组覆膜处与不覆膜处的黏膜增殖细胞阳性表达有显著的统计学差异(P<0.05)。不覆膜处的黏膜增殖细胞比率较高。结论①气管支架有无覆膜在留置的短期内对于进食、呼吸状况及痰液的排出无明显影响;②气管支架留置后,覆膜段气管内膜组织增生水平要小于不覆膜段;③气管支架留置后,覆膜段的黏膜增值细胞阳性表达小于不覆膜段。覆膜支架的生物相容性好。Objective On the basic research of different intra-tracheal stent placement in dogs, we try to further explore the application value of membrane covered stent. Methods Totally 18 healthy, matured and hybrid dogs were randomly divided into 3 groups with different type of intra-tracheal stent placement. Group A （n = 6）： with whole membrane covered stent （6 cm, length covered） placement. Group B （n = 6）： with middle segment membrane covered stent （4 cm in length with either lcm uncovered at the upper and lower terminal）. Group C（n = 6）： with uncovered stent （6 cm in length）. After intra-tracheal stent placement, all dogs were monitored with recording of clinical assessment of food intake, expectoration and respiration. Twenty weeks later, all dogs were sacrificed and their tracheal specimen were inspected grossly and HE examination. The further investigation included PCNA stain, the positive expression of proliferative cells by quantification analysis. Results After intra-tracheal stent placement, all dogs showed no acute dyspnea but with a mild to moderate cough with no obvious difference in expectoration. All animals were normal in drinking, taking food and bark, and only a part of them seemed to be in low activity. The proliferation of endotracheal wall was more prominent at the non-membrane covered stent placement sites, together with more severe sterotic lumens than those of the membrane covered stent placement sites. All groups demonstrated individually the inflammatory change, squamous metaplasia of low columnal epithelial cells, granulomatosis and fibrous proliferation under HE stain examination. The positive expression of mucosal proliferative cells involved all the groups showed statistical significant differences （P 〈 0.05）, with higher preoportional rates for non-membrane covered stent placement site. Conclusion Short term intra-tracheal placement with membrane covered or non-covered stent shows no significant influence clinically. The outcome of membrane covered stent

关 键 词：气管支架 覆膜 动物实验 

分 类 号：R562[医药卫生—呼吸系统]