混合尿液质粒缺口-连接酶链反应-酶联免疫吸附法检测孕妇沙眼衣原体感染  

Pooling urine plasmid gap-LCR-ELISA to detect Chlamydia trachomatis infection in pregnant women

作　　者：伍金林[1] 韦红[2] 吴仕孝[2] 

机构地区：[1]四川大学华西第二医院新生儿科,成都610041 [2]重庆医科大学附属儿童医院新生儿科

出　　处：《中华妇幼临床医学杂志（电子版）》2007年第4期194-196,共3页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家自然科学基金资助项目(基金编号:30200300)

摘　　要：目的建立高效的围生期沙眼衣原体(Chlamydia trachomatis,CT)流行病学调查批处理方法--混合尿液(pooling urine)质粒缺口-连接酶链反应-酶联免疫吸附法(gap-ligase chain reaction-en-zyme linked immunoabsorbent assay,gap-LCR-ELISA)。方法比较质粒gap-LCR-ELISA对不同体积孕妇尿液混合库(4×,6×,8×,10×)及单份样本(1×)检出CT感染的灵敏度和特异度。结果质粒gap-LCR-ELISA检测4,6,8,10人份混合尿样中的CT感染均可达到100%的特异度,灵敏度分别为95%,85%,70%和65%,各组实验成本下降的比例均超过40%。结论4人份孕妇混合尿液质粒gap-LCR-ELISA的检测效能,等同于对单份标本的独立检测,为该法最适宜的标本混合策略,在大规模流行病学调查中具有应用价值。Objective To construct an effective pooling urine plasmid gap-LCR-ELISA method for large-scale epidemic screening of perinatal Chlamydia trachoraatis （CT） infection. Methods The most optimal pooling size （4×, 6× , 8×, 10× ） was chosen by comparing the sensitivity, specificity and costeffectiveness with single urine detection. Results Pooling urine by 4 ×, 6 ×, 8 ×, 10 × size to gap-LCR- ELISA resulted in a satisfying specificity up to 100%, but the sensitivity declined with the increase of pooling volume （95%, 85%, 70% and 65%, respectively）. And a significant cost reduction of more than 40% was obtained in all the groups. Conclusion 4× size pooling urine strategy could achieve the same detecting capability as that of individual specimen examined by plasmid gap-LCR-ELISA, which is quite valuable in large scale epidemic screening for CT infection.

关 键 词：沙眼衣原体 质粒 缺口-连接酶链反应-酶联免疫吸附法 尿 

分 类 号：R714.2[医药卫生—妇产科学]