高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质  

Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：田晨煦[1] 徐小平[1] 廖丽云[2] 张洁[1] 刘静[1] 周莎[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]成都医学院,四川成都610083

出　　处：《色谱》2007年第4期496-500,共5页Chinese Journal of Chromatography

基　　金：四川省技术创新重点项目(批准文号:成财建[2007]53号)

摘　　要：建立了高效液相色谱-串联质谱法(HPLC-MS/MS)分离和鉴定绿原酸及其相关杂质的方法。采用C18色谱柱(5μm,4.6mm×150mm),乙腈-水(含0.1%甲酸)(体积比为8∶92)为流动相,经HPLC-MS/MS和HPLC-二极管阵列检测器在线检测,对工业绿原酸中的奎尼酸、咖啡酸、绿原酸同分异构体等8个相关杂质的结构进行了鉴定。A method using high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） was established for analysis and identification of chlorogenic acid along with its related impurities. A Gemini C18 HPLC column （4.6 mm× 150 mm,5 μm） was used with acetonitrile-water （ containing 0.1% formic acid） （8： 92, v/v） as mobile phase. Eight related impurities of chlorogenic acid were identified and their structures were determined by using online HPLC-MS/MS and photodiode array detector （DAD）. The method is quick, simple and can be used directly to identify the structure of unknown trace substances in the sample of chlorogenic acid.

关 键 词：高效液相色谱-串联质谱法 绿原酸 相关杂质 鉴别 

分 类 号：O658[理学—分析化学]