高效液相色谱法同时测定长春花中的文多灵、长春质碱和脱水长春碱  

Simultaneous Determination of Vindoline,Catharanthine and Anhydrovinblastine in Catharanthus roseus by High Performance Liquid Chromatography

作　　者：杨蕾[1] 唐中华[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040

出　　处：《色谱》2007年第4期550-552,共3页Chinese Journal of Chromatography

基　　金：教育部重点项目(No.104191)

摘　　要：运用高效液相色谱（HPLC）梯度洗脱同时测定了长春花中的文多灵、长春质碱和脱水长春碱等3种生物碱。色谱柱为Waters5C18-MS-Ⅱ（4.6mm×250mm）,流动相为甲醇-1%二乙胺（磷酸调pH至7.3）系统,梯度洗脱,检测波长220nm,柱温25℃。实验结果表明,文多灵、长春质碱和脱水长春碱等3种生物碱分别在0.03-1mg/mL,0.03-1mg/mL和0.01-0.5mg/mL时线性关系良好,平均加样回收率分别为96.8%,97.0%和96.4%,相对标准偏差（RSD）分别为1.53%,1.37%和1.96%。用该法同时检测了不同干旱条件处理下长春花样品中的文多灵、长春质碱和脱水长春碱,方法准确、快速、简便。A high performance liquid chromatographic （HPLC） method with gradient elution was developed for the simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus. The analytes were separated on a Waters 5C18-MS- Ⅱ column （4.6 mm ×250 mm） and detected at 220 nm. The mobile phases were methanol-l% （v/v） diethylamine solution （ adjusted to pH 7.3 with phosphate） for gradient elution at temperature of 25℃（3. The calibration curves for vindoline, catharanthine and anhydrovinblastine showed good linearity in the ranges of 0.03 - 1 mg/mL （ r = 0. 999 7 ）, 0.03 - 1 mg/mL （ r = 0. 999 9 ） and 0.01 -0. 5 mg/mL （r = 0. 998 6）, respectively. The recoveries were 96.8%, 97.0%, and 96.4% and the relative standard deviations （RSDs） were 1.53%, 1.37%, and 1.96%. The method is accurate, rapid and simple for the determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus.

关 键 词：高效液相色谱法 文多灵 长春质碱 脱水长春碱 长春花 

分 类 号：O658[理学—分析化学]