神经元原代培养方法的改进  

Improved Methods of Primary Cultured Neurons

作　　者：郭琴[1] 毛萌[1] 罗小丽[1] 孙小妹[1] 李胜富[2] 周晖[1] 

机构地区：[1]四川大学华西第二医院儿科 [2]四川大学华西医院移植工程与免疫实验室,四川成都610041

出　　处：《华西医学》2007年第3期566-568,共3页West China Medical Journal

基　　金：国家自然科学基金资助(30400478)

摘　　要：本研究主要讨论近年来本课题组不断改进体外神经元培养方法以获得高纯度高活性的大鼠皮质神经元的研究结果。结果表明,联合使用低浓度胰酶及DNaseⅠ进行消化,在培养基的组成成分中用Glutamax代替glu-tamine,并将胎牛血清比率降为5%,于接种后48小时加入细胞分裂抑制剂-阿糖胞苷以抑制神经胶质细胞生长,可获得高纯度高活性的大鼠皮质神经元。Objective： To obtain rat＇ s cortical neurons with high purity and high viability in vitro, we have been investigating and improving the methods for years. The results showed that cortical neurons of rat with high purity and activity can be harvested by using low concentrations of trypsin and DNase I for digestion, and glutamax instead of glutamine in the composition of the medium. At the same time, the percentage of the fetal calf serum is reduced to 5% and cell division inhibitor- Ara - C added to the medium to inhibit growth of ghal cells at 48 hours after cultivation.

关 键 词：原代培养 大鼠皮质神经元 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]