人脐带间充质干细胞向心肌样细胞分化的研究  

Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells into Cardiomyocytes

作　　者：林晓波[1] 何红燕[2] 罗敏洁[1] 崔冰琳[1] 冯学永[1] 蒋学武[1] 谢庆东[3] 黄天华[3] 应文娟[2] 马廉[1] 

机构地区：[1]汕头大学医学院第二附属医院儿科,515041 [2]汕头大学医学院第一附属医院外科,515041 [3]汕头大学医学院生殖医学研究中心,广东汕头515041

出　　处：《实用儿科临床杂志》2007年第13期971-973,977,共4页Journal of Applied Clinical Pediatrics

基　　金：广东省社会发展计划项目资助(2005B30601013)

摘　　要：目的探讨人脐带间充质干细胞(MSCs)向心肌样细胞分化可能性,为心肌细胞移植探索新细胞来源。方法采用5-氮杂胞苷(5-Aza)和二甲基亚砜(DMSO)诱导人脐带间充质干细胞分化。观察诱导后分化细胞形态变化,用免疫组织化学方法检测分化心肌样细胞的心肌肌钙蛋白I(troponin I)、心肌肌钙蛋白T(troponin T)和心肌结蛋白(desmin),逆转录聚合酶链反应(RT-PCR)测定分化的心肌样细胞是否有细胞心肌特异转录因子(Nkx2.5)和心肌细胞desmin的cDNA表达。结果人脐带MSCs经5-Aza和DMSO诱导后可向心肌样细胞分化,分化细胞表达心肌细胞的标记troponinI、troponinT和desmin。RT-PCR检测证实,人脐带MSCs诱导前不表达Nkx2.5和desmin,经5-Aza和DMSO诱导后表达心肌细胞标记Nkx2.5和desmin。结论人脐带间充质干细胞可以分化为心肌样细胞,5-Aza和DMSO可作为心肌细胞诱导剂,人脐带间充质干细胞可以作为心肌细胞的来源。Objective To investigate human umbilical cord mesenchymal stem cells（MSCs） differentiated into cardiomyocytes,and find a new source of cells for transplantation of cardiomyocytes. Methods MSCs were induced by incubated 5 - azacytidine （ 5 - Aza） and dimethylsulfoxide（DMSO）. The myogenic cells differentiated were then evaluated by immunohistochemistry stained for desmin, cardiac troponin I and cardiac treponin T. The method of RT - PCR was used to test if the cells induced could express Nkx2.5 and desmin cDNA. This new cell line provided a powerful model for the study of cardiomyocyte differentiation. Results The MSCs derived from human umbilical cord could differentiate into cardiomyocytes induced by both 5 - Aza and DMSO. Immunocytochemistry showed that differentiated cardiomyocyte - like cells expressed both desmin, cardiac troponin I and cardiac troponin T positive. The cells expressed Nkx2.5 and desmin cDNA after induced. Conclusions The MSCs derived from human umbilical cord Wharton＇s Jelly can differentiate into cardiomyocytes induced by both 5 - Aza and DMSO. Based on these observations, and taking into account immense advantages of UCB over than other sources of stem cell,human umbilical cord - derived MSCs retain mvocardiogenic potential suitable for cell theranv and transplantation against intractable heart diseases.

关 键 词：脐带 间充质干细胞 心肌样细胞 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]