苏云金芽孢杆菌菌株B-Hm-16的生物学特性及cry基因型鉴定  

Biological Characteristics of Bacillus thuringiensis strain B-Hm-16 and identification of its cry-type genes

作　　者：李长友[1] 李国勋[1] 张杰[2] 宋福平[2] 黄大昉[2] 

机构地区：[1]青岛农业大学植物保护学院,山东青岛266109 [2]中国农科院植保所植物病虫害生物学国家重点实验室

出　　处：《青岛农业大学学报（自然科学版）》2007年第1期1-4,共4页Journal of Qingdao Agricultural University(Natural Science)

基　　金：973计划项目(2003CB114201);莱阳农学院博士启动基金资助

摘　　要：B-Hm-16是我室从患病昆虫体内分离的一株苏云金芽孢杆菌,对小菜蛾、甜菜夜蛾、棉铃虫、粘虫和甘蓝夜蛾等多种害虫均具有较高活性;主要形成钝菱形晶体,杀虫蛋白晶体主要由130kDa的蛋白组成;酯酶型与标准菌株蜡螟亚种(B.t.subsp.galleriae)和库斯塔克亚种(B.t.subsp.kurstaki)一致,属于galleriae型;10项生理生化反应结果同标准菌株鲇泽(B.t.subsp.aizawai)亚种一致;经PCR-RFLP鉴定该菌株含有cry1Ab和cry9Ea基因,不含有cry2、cry3、cry4、cry5、cry8和cry11等基因。Bacillus thuringiensis strain B-Hm-16 was isolated from diseased insect in China. It＇s toxicity against Plutella xylostella, Spodoptera exigua, Helicoverpa armigera, Mythimna separata and Mamestra brassicae. The strain B-Hm-16 produced crystals composed of 130 kDa proteins. Results of esterase pattern identification showed that B-Hm-16 was the same as standard strains of B. t. subsp, galleriae and B. t. subsp, kurstaki. Biochemical reaction indicated that B-Hm-16 was identical with B. t. subsp, aizawai. Results of cry - type gene identification showed that B-Hm-16 contained crylAb and cry9Ea genes, but it did not contain cry2, cry3, cry4, cry5, cry8 and cryl 1 genes.

关 键 词：苏云金芽孢杆菌 杀虫活性 酯酶型 生理生化反应 基因鉴定 

分 类 号：Q939.96[生物学—微生物学]