血管生成抑制素质粒治疗子宫内膜异位症的实验研究  

The therapeutic effect of angiostatin plasmid on a murine model of endometriosis

作　　者：陈伟芳[1] 张江宇[2] 范保维[1] 罗喜平[1] 

机构地区：[1]广东省妇幼保健院妇科,广州市510010 [2]广东省妇幼保健院病理科,广州市510010

出　　处：《实用医学杂志》2007年第14期2126-2128,共3页The Journal of Practical Medicine

基　　金：广东省医药卫生科学研究基金资助项目(编号:B2004006)

摘　　要：目的:探讨血管生成抑制素(angiostatin)质粒治疗大鼠子宫内膜异位症的效果。方法:用手术种植内膜方法建立大鼠子宫内膜异位症模型,将已成模大鼠随机分为3组,对照组(n=15)、生理盐水组(n=15)和治疗组(n=15),对照组常规饲养,生理盐水组和治疗组分别在病灶内注射生理盐水血管生成抑制素质粒(每只50!g/0.1mL),1周后重复注射1次,第2次治疗后1周处死3组大鼠。测量异位病灶的生长情况,观察病灶的病理组织学改变和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达情况。结果:治疗组的异位病灶体积缩小,VEGF的表达与生理盐水组及对照组比较差异均有显著性(P<0.05)。结论:血管生成抑制素的基因治疗使大鼠子宫内膜异位病灶体积缩小,VEGF的表达减少,对大鼠子宫内膜异位症有治疗作用。Objective To explore the therapeut.ic effect of angiostatin plasmid on a murine model of endometriosis. Methods A murine model of endometriosis was established by autotransplantation with uterine tissues. Four weeks after autotransplantation, the rats were randomly assigned to receive routine feeding （control group, n=15）, an intrafocal injection of normal saline （NS group,n=15）, or an intrafocal injection of angiostatin plasmid （50 μg/0.1 mL for each; angiostatin group,n=15）. The injection was repeated one week later. All rats were then executed one week following the second injection. The growth of implants was measured. Pathological changes and vascular endothelial growth factor （VEGF） were detected in the ectopic endometrium. Results Implant volume was significantly reduced in the angiostatin group. The VEGF expression of ectopic endometrium in the angiostatin group was significantly lower than that in the NS group or the control group （P 〈 0.05）. Conclusion Gene therapy with angiostatin is an effective treatment for rat endometriosis.

关 键 词：子宫内膜异位症 血管生成抑制剂 动物模型 基因治疗 

分 类 号：R711.71[医药卫生—妇产科学]