银杏天宝对大鼠实验性结肠炎抗氧化作用的影响  

Effects of Ginkgo biloba extract on the antioxidation in rats with TNBS-induced colitis

作　　者：周燕红[1] 于皆平[2] 

机构地区：[1]咸宁学院医学院消化内科,湖北省咸宁市437100 [2]武汉大学人民医院消化内科,湖北省武汉市430060

出　　处：《世界华人消化杂志》2007年第15期1701-1705,共5页World Chinese Journal of Digestology

基　　金：湖北省教育厅资助项目;No.B200528003~~

摘　　要：目的:在三硝基苯磺酸灌肠诱导大鼠实验性结肠炎模型中,研究银杏天宝(EGB)的治疗作用及其抗氧化损伤作用的机制.方法:应用三硝基苯磺酸(TNBS)/乙醇灌肠制备大鼠实验性结肠炎模型.实验设正常对照组,三硝基苯磺酸模型组,阳性药物对照组(5-ASA group,100 mg/kg),EGB组(200 mg/kg) 4组.观察大鼠肠组织大体形态和组织学评分.生化法检测大鼠肠组织超氧化物歧化酶(SOD),谷胱苷肽过氧化物酶(GSH-Px)活性及丙二醛(MDA),一氧化氮(NO)含量.免疫组化检测肠组织诱导型一氧化氮合酶(iNOS)蛋白的表达.结果:与模型组相比,EGB组结肠组织iNOS表达明显减少,NO、MDA明显降低(iNOS:19.60%±3.17% vs 81.36%±1.71%;NO:9.20±0.81μmol/g vs 14.77±1.34μmol/g;MDA:3.96±0_35 umol/g vs 6.06±0.39 umol/g;P<0.01):SOD、GSH-Px活性明显升高(SOD:32.52±1.82 kU/g vs 21.90±2.22 kU/g;GSH- Px:49.91±2.59 kU/g vs 41.26±2.90 kU/g;P<0.01).EGB能明显减少大鼠实验性结肠炎模型组大体形态和组织学评分(2.10±0.57vs 3.10±0.57:3.50±0.85 vs 4.7±0.82;P<0.01).结论:EGB可能通过抑制氧自由基反应,抗氧化损伤,抑制NO生成,来减轻结肠炎炎症反应.AIM： To investigate the therapeutic effect and antioxidation mechanism of Ginkgo biloba extract （EGB） in rats with ulcerative colitis induced by 2, 4, 6-trinitrobenzene sulfonic acid （TNBS）. METHODS： Rat model of ulcerative colitis was induced by 2, 4, 6-trinitrobenzene sulfonic acid （150 mg/kg） with ethanol. The experimental animals were randomly divided into 4 groups： normal group, model group, 5-arninosalicylic acid （5-ASA, 100 mg/kg） group, EGB （200 mg/kg） group. The macroscopic and histological damages were evaluated. The contents of malondiadehyde （MDA）, nitric oxide （NO）, superoxide dismutase （SOD）, and glutathion peroxide （GSH- Px） were measured by biochemical methods. The expression of inducible nitric oxide synthase （iNOS） in the colon tissues was detected by immunchistochemistry. RESULTS： The expression of iNOS （19.60% ± 3.17% vs 81.36% ± 1.71%）, the tissue contents of NO （9.20 ± 0.81 μmol/g vs 14.77 ± 1.34μmol/g, P 〈 0.01） and MDA （3.96 ± 0.35μmol/g vs 6.06 ± 0.39μmol/g, P 〈 0.01） in EGB group were obviously lower than those in model group, but the activities of SOD and GSH-Px of colon tissues in EGB group were significantly higher （SOD： 32.52 ± 1.82 kU/g vs 21.90 ± 2.22 kU/g; GSH-Px： 49.91± 2.59 kU/g vs 41.26 ± 2.90 kU/g; P 〈 0.01）. EGB also notably reduced the macroscopic and histological scores of colon tissues （2.10 ± 0.57 vs 3.10 ± 0.57; 3.50 ± 0.85 vs 4.7 ± 0.82; both P 〈 0.01）. CONCLUSION： EGB can alleviate the inflammatory reactions in colitis through inhibiting oxygen free radical reaction, exerting antioxidation effects and decreasing the production of NO.

关 键 词：银杏天宝 溃疡性结肠炎 氧自由基 抗氧化 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]