LC-MS法测定山楂叶中多元酚类成分的含量  

Determination of the principal polyphenolic components in the leaves of Crataegus L. by LC-MS

作　　者：江爱龙[1] 刘荣华[2] 邵峰[2] 马志林[2] 余伯阳[3] 

机构地区：[1]武警江西总队医院医学工程科,江西南昌330030 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [3]中国药科大学中药复方研究室,江苏南京210038

出　　处：《武警医学院学报》2007年第5期533-537,543,共6页Acta Academiae Medicinae CPAPF

基　　金：国家自然科学基金资助项目(30472161);江西省自然科学基金资助项目(0640163)

摘　　要：【目的】利用LC-ESI-MS法测定不同山楂叶中8种主要多元酚类成分的含量。【方法】采用Lichro-spherC18(250×4.6mmI.D.,5μm)色谱柱;流动相A为乙腈,B为(0.5%甲酸溶液),梯度洗脱,0～12minA由11%～17%,12～30minA由17%～18%,30～45minA由18%～40%,45～60minA由40%～100%;流速为1ml/min,三向分流阀分流进入MSD进行检测和VWD进行监测;柱温30℃。【结果】8种多元酚类成分在不同种、不同产地及不同采收期的山楂叶中的含量存在明显的差异。【结论】本方法可用于山楂叶中多元酚类成分的测定。[Objective] To determine the content of eight principal polyphenolic components in hawthorn leaves by LC - ESI- MS. [Methods] The system of lichrospher C,s column （250 ×4.6mm I. D., 5 μm）, mobile phase consisted of solvent A （acetonitrile） and solvent B （0.5% formic acid）, elution profile： 0 - 12 min 11% to 17% A in B, 12 - 30 min 17% to 18% AinB, 30-45 min 18% to 40% AinB, 45-60 min 40% to 100% AinB; flow rate： 1.00ml/ min was adopted and flowed into MSD and VWD by diffluence, column temperature 30℃, [Results] The main polyphenolic components in hawthorn leaves were different among the different species, locations and growth stages. [Conclusions] This method can be used for the determination of the main polyphenolic components in hawthorn leaves.

关 键 词：黄酮 山楂叶 LC-MS 多元酚 药物分析 

分 类 号：R927.2[医药卫生—药学]