三羟异黄酮对去卵巢大鼠主动脉雌激素β受体和原癌基因c-fos表达的影响  

Effects of Genistein on The Expression of Estrogen Receptor β and c-fos Gene in Aorta in Ovariectomized Rat

作　　者：张秀荣[1] 樊秀云[1] 史立宏[1] 刘跃春[1] 

机构地区：[1]潍坊医学院省应用药理学重点实验室,山东潍坊261042

出　　处：《中华高血压杂志》2007年第7期570-574,共5页Chinese Journal of Hypertension

基　　金：山东省卫生厅医学科研项目(No2001CA1CCA2)

摘　　要：目的观察三羟异黄酮(GST)对去卵巢大鼠主动脉雌激素β受体(ERβ)和原癌基因c-fos表达的影响。方法将成年雌性大鼠40只随机分为4组:假手术对照组(A组);去卵巢组(B组);去卵巢+雌二醇组(C组);去卵巢+GST组(D组)。给药8周后处死大鼠。免疫组化法检测主动脉中ERβ和原癌基因c-fos的表达。结果1)去卵巢手术大鼠主动脉ERβ的表达明显降低,GST与雌二醇明显增加去卵巢大鼠主动脉ERβ的表达。2)去卵巢手术增加大鼠主动脉原癌基因c-fos的表达,但GST与雌二醇能阻止c-fos表达增加。结论GST对血管ERβ的表达具有调节作用;GST可抑制血管平滑肌原癌基因c-fos的表达。这可能是植物雌激素防止动脉粥样硬化,发挥心血管保护作用的机理之一。Objective To study the effects of genistein （GST） on the expression of estrogen receptor β （ERβ） and the expression of c-fos gene in the aorta of ovariectomized rats. Methods Forty female Wistar rats were randomly divided into four groups： sham-operated （control）, ovariectomized （OVX）, ovariectomized with 17β-E2 replacement （OVX ＋ E2 ）, ovariectomized with genistein replacement （ OVX ＋ GST） group. After 8 weeks＇ replacement therapy, the rats were sacrificed and the expression of ERβ and c-fos in the aorta was studied by immunohistochemistry. Results Ovariectomy significantly decreased ERβ expression and increased c-fos ex-pression in aorta; while replacement therapy, GST and E2, attenuated the effect of ovariectomy manifested by in-creasing ER expression and decreasing c-fos expression in aorta. Conclusion GST may modulate the expression of ERβ in the arteries of ovariectomized rats and play a beneficial effects on cardiovascular system which was asso-ciated with decreases in expression of c-fos gene in aorta.

关 键 词：三羟异黄酮 雌二醇 雌激素Β受体 原癌基因C-FOS 动脉粥样硬化 

分 类 号：R96[医药卫生—药理学]