不同生育期菊芋叶片过氧化物酶同工酶表达特点的研究  

Study on expression characteristics of jerusalem artichoke(Helianthus tuberosus.) leaves peroxidase isoenzymes in various growth periods

作　　者：陈燕珍[1] 胡丽莲[1] 陶毅明[1] 梁杨琳[1] 

机构地区：[1]广西师范大学生命科学学院,桂林541004

出　　处：《生物学杂志》2007年第1期25-27,46,共4页Journal of Biology

基　　金：广西师范大学生态学博士点建设项目(XKY2006ZD02-子课题)

摘　　要：用聚丙烯酰胺凝胶电泳(PAGE)法,分离了不同生育期菊芋叶片过氧化物酶(Helianthus tuberosus.1eves peroxidase,HLP)同工酶,并测定了HLP的酶活性;比较了菊芋叶及其他4种植物POD的特点。结果表明:(1)同一植株不同部位的HLP酶活性大小顺序为,中部成熟叶(6.89×103U/g)>底部衰老叶(5.38×103U/g)>上部嫩叶(2.82×103U/g);由嫩叶→成熟叶→衰老叶的发育过程中,HLP同工酶酶带共有9条,出现的方式有稳定型(Rf 0.81带)、酶活性先弱后强型(Rf0.52、0.67、0.74、0.85带)、酶带先有后无型(Rf 0.70、0.72带)、酶带先无后有型(Rf 0.77)4种。(2)几种植物POD的酶活性的大小顺序是:甘蔗叶>竹笋壳>菊芋叶>大豆壳;POD同工酶电泳最大迁移率(Rf)大小顺序是:菊芋叶(0.85)>甘蔗叶(0.70)>竹笋壳(0.61)>大豆壳(0.57)>辣根(0.55),HLP同工酶属阴离子型。探讨了菊芋POD的表达特点。Peroxidase isoenzymes of jerusalem artichoke （Helianthus tuberosus. ） leaves in various growth periods were seperated by means of PAGE. Peroxidase enzyme activities of jerusalem artichoke leaves in various periods were determined and compared with POD of other 4 plants. It was found that POD enzyme activities in different parts of the plant were ordered as follow： mature leaves in the mid part （6.89× 10^3U/g） 〉 old leaves in the lower part （5.38× 10^3U/g） 〉 new leaves in the upper part （2. 82 × 10^3U/g） ;9 POD isoenzymes bands appeared in the growth process from new leaves, mature leaves to old leaves. Four kinds of appearances were included： band of Rf 0.81 was stable; isoenzymes activities of bands of Rf 0. 52, 0.67, 0.74, 0.85 first weak then strong; band of Rf 0.70, 0.72 appeared first then disappeared; band of Rf 0. 77 disappeared first then appeared. The POD enzymes activities were ordered as follow, cane leaves 〉 bamboo shell 〉 jerusalem artichoke leave 〉 soybean hull; Rf values of POD isoenzymes were as followed： jerusalem artichoke leave （0. 85） 〉 cane leave （0.70） 〉 bamboo shell （0.61） 〉 soybean hull （0.57） 〉 horseradish （0. 55）. jerusalem artichoke peroxidase isoenzyme is of negative ion. The characteristics of jerusalem artichoke POD - isoenzymes expression were also discussed.

关 键 词：菊芋 过氧化物酶 同工酶 不同生育期 

分 类 号：Q554.6[生物学—生物化学]