ASE-HPLC法测定黄姜中薯蓣皂甙元的含量  

RESEARCH ON DETERMINATION OF DIOSGENIN IN DIOSCOREA ZINGIBERENSIS BY ASE-HPLC

作　　者：贾林[1] 张皋[1] 刘红妮[1] 胡玲[1] 张翠娥[1] 刘敏[1] 

机构地区：[1]西安近代化学研究所,西安710065

出　　处：《化学分析计量》2007年第4期18-20,共3页Chemical Analysis And Meterage

摘　　要：采用ASE200快速溶剂萃取机提取技术和高效液相色谱法,测定黄姜中薯蓣皂甙元的含量。确定了ASE200提取条件以及HPLC测定条件,以甲醇作为流动相,流速1mL/min,UV检测器,测定波长210nm,采用YWGC18柱(150mm×4.6mmi.d.,10μm)。测定皂甙元的线性范围为0.01～2.93g/L,r=0.9996,样品检出限为0.2mg/L,加标回收率为97.1%～99.8%,RSD=1.1%(n=7)。The method for determination of diosgenin in dioscorea zingiberensis was established. The dioscorea samples were extracted by ASE200 and determinated by HPLC. The analytical column was YWG Cls （150 mm×4.6 mmi. d. , 10μm）. The mobile phase was methanol with flow rate of 1.0 mL/min. The UV detector wavelength was 210 nm. There was a good linear relationship in the range of 0.01 -2.93 g/L（r =0. 9996）. The sample detection limit was 0.2 mg/L. The sample recoveries were in the range of 97.1% -99.8% and RSD was 1.1% （n =7）.

关 键 词：黄姜 薯蓣皂甙元 快速溶剂萃取机 HPLC 

分 类 号：O657.72[理学—分析化学]