以多胺为探针的DNA共振光散射光谱法测定  

Resonance Scattering Spectrometric Determination of DNA with Polyamine as Probe

作　　者：冯硕[1] 李正平[1] 吴秋华[1] 方正[1] 张淑红[1] 

机构地区：[1]河北农业大学理学院,保定071001

出　　处：《理化检验（化学分册）》2007年第7期555-557,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：研究了三种常见多胺与小牛胸腺脱氧核糖核酸（DNA）作用的共振光散射光谱。发现在PH2．21～9．15的BR缓冲溶液中，小牛胸腺DNA与精胺的结合产生强烈的共振光散射，散射强度在343nm波长处达到最大。小牛胸腺DNA的质量浓度在0．1～40mg·L^-1范围内与共振光散射强度呈线性关系，回归方程的相关系数为0．9989，检出限为19μg·L^-1。5倍于DNA浓度的蛋白质、核苷酸都不干扰DNA的分析测定，金属离子的允许浓度一般为1.0×10^-6mol．L^-1．The interaction of DNA with three polyamines was studied by resonance scattering spectrometry. It was found that the intensity of resonance scattering of spermine was greatly enhanced by calf thymus DNA in a Britton-Robinson buffer of pH range of 2. 21 - 9. 15. A resonance scattering peak was found at 343 nm. The concentration of calf thymus DNA in the range of 0. 1-40 mg · L^-1 was proportional to the enhanced resonance scattering intensity, with a correlation coefficient of 0. 998 9. The detection limit was found to be 19 μg . L^-1. The proteins at 10 mg . L^-1 , nucleotides at 10 mg . L^-1 did not interfere with the determination of 2. 0 mg . L^-1 DNAs. Most of the common metal ions at 1. 0 × 10^-6 mol . L^-1 were tolerable. Based on these facts a method for determination of DNA by resonance scattering spectrometry was proposed.

关 键 词：DNA 共振光散射光谱 多胺 精胺 

分 类 号：O657.3[理学—分析化学]