非水介质毛细管电泳高频电导法测定姜黄中姜黄素  

Determination of Curcumin in Curcuma by Capillary Electrophoresis with High Frequency Conductivity Detection in Nonaqueous Medium

作　　者：徐健君 翟海云[2] 陈缵光[3] 林伟丰[2] 蔡沛祥[2] 莫金垣[2] 

机构地区：[1]珠海市质量计量监督检测所,珠海519000 [2]中山大学化学与化学工程学院,广州510275 [3]中山大学药学院,广州510089

出　　处：《理化检验（化学分册）》2007年第7期588-590,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(20375049和20575080)

摘　　要：建立了药材姜黄中姜黄素含量的非水介质毛细管电泳高频电导测定方法。对非水介质体系和支持电解质的种类，浓度以及操作电压和进样时间等影响因素进行了优化。以无水甲醇作为分离介质，NH4OAc-HOAc为电解质，25．0kV为分离电压，可在12min内实现对姜黄素的分离检测。在最佳试验条件下，姜黄素的线性范围为3．0-140．0mg·L^-1，检出限为0．5mg·L^-1，回收率为95．3％～100．9％．It was reported in this paper that curcumine was separated from curcuma and determined by capillary electrophoresis with high-frequency conductivity detection in non-aqueous medium within 12 rain. Optimum conditions for the analysis was described as follows： （1） separation medium： anhydrous methanol; （2） electrolyte： 6 mmol · L^-1 HOAc＋8 mmol· L^-1 NH4OAc solutions （pH 4. 5）; （3） separation voltage. 25. 0 kV. Linear relationship was kept between peak area and concentration of curcumine in the range from 3.0 to 140. 0 mg per L of solution （r=0. 997）, with a detection limit of 0. 5 mg · L^-1. Samples of curcuma grown in Sichuan, Guangdong and Guangxi were collected and analyzed by the proposed method. Values of recovery test were in the range from 95.3%-100. 9%.

关 键 词：非水介质毛细管电泳 高频电导检测 姜黄素 姜黄 

分 类 号：O657.31[理学—分析化学]