不同阳离子皂土对植物RNA提取效果的影响  

Effect of the Different Cation-Bentonites on the Preparation of Plant RNAs

作　　者：邢杰[1] 淳俊[1] 郑彦峰[1] 王胜华[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064

出　　处：《安徽农业科学》2007年第21期6385-6385,6390,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(104255);四川大学科研启动基金

摘　　要：在提取缓冲液中加入皂土能有效地去除蛋白质并抑制RNAse,以Mg2+、K+和Na+3种阳离子处理的皂土提取缓冲液提取麻疯树叶片总RNA,结果显示,不同的处理对RNA的提取效果明显不同。其中Mg2+-K+-Na+-皂土的提取效果最佳,叶片RNA提取得率为790μg/g(鲜重),比对照高6倍。用该法提取的RNA能用于分子克隆与基因表达分析等后继分子生物学实验。该方法为一种简便,快速,经济高效的RNA提取方法。Bentonite was added in extraction bufer in order to get rid of protein and restrain RNAs. The bentonites worked with cations strongly enhanced isolation of total RNAs from the leaf of Jatropha curcas and there were different extraction efficiencies in different cafion-bentonites and the Mg^2 ＋ - K^ ＋ - Na^＋ -bentonite was the best one, with 790 μg/g RNA of the fresh leaf, 6 times higher than the reference. This method can meet the needs of most molecular biological experiments includ/ng gene cloning and expression analysis.

关 键 词：RNA提取 麻疯树 阳离子 皂土 

分 类 号：Q946[生物学—植物学]