复方银黄微型灌肠剂对发热大鼠下丘脑中COX-1、COX-2蛋白表达的影响  

EFFECT OF THE COMPOUND OF YINHUANG MICRO-ENEMA ON THE EXPRESSION OF COX-1 AND COX-2 PROTEINS IN HYPOTHALAMUS IN FEVER RATS

作　　者：杨斌[1] 刘华钢[1] 阳洁[1] 赖茂祥[2] 

机构地区：[1]广西医科大学药学院药理学教研室,南宁530021 [2]广西中医药研究所,南宁530022

出　　处：《广西医科大学学报》2007年第3期335-337,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No3026124)

摘　　要：目的:研究复方银黄微型灌肠剂(compound of Yinhuang micro-enema,YHCE)对发热大鼠下丘脑中环氧化酶-1(cyclooxygenase,COX-1)、环氧化酶-2(cyclooxygenase,COX-2)蛋白表达的影响,探讨YHCE的解热作用机制。方法:用酵母混悬液复制发热大鼠模型,采用免疫组化ElivisionTMplus二步法观察下丘脑中COX-1、COX-2蛋白表达的情况。结果:YHCE能明显抑制发热大鼠的体温上升,且能抑制下丘脑中COX-2蛋白表达,而对下丘脑中COX-1蛋白表达无明显影响。结论:YHCE有明显的解热作用,其解热作用机制可能与抑制发热大鼠下丘脑中COX-2蛋白表达有关。Objective： To study the effect of the compound Yinhuang micro-enema（YHCE） on the expressions of COX-1 and COX-2 proteins in hypothalamus in fever rats and to explore its mechanism. Methods： The animal model was induced by injected suspension of yeast hypodermically. The expressions of COX-1 and COX-2 proteins in hypothalamus were detected by Elivision^TM plus method of immunohistochemistry. Result： YHCE inhibited significantly the temperature rising and the expression of COX-2 protein, but it had no obvious effect on the expression of COX-1 protein. Conclusion： YHCE has obvious antipyretic effect and its mechanism may be related to its inhibition of the expression of COX-2 protein in hypothalamus.

关 键 词：复方银黄微型灌肠剂 解热 COX 免疫组化 

分 类 号：R285.5[医药卫生—中药学]