PKC和MMPs在枸杞籽油抗动脉粥样硬化中的作用和机制  

Anti-atherosclerosis potency by Lycium seed oil and its possible mechanism of PKC and MMPs

作　　者：姜怡邓[1] 曹军[1] 董泉洲[1] 王树人[2] 

机构地区：[1]宁夏医学院基础学院,宁夏银川750004 [2]四川大学华西基础医学与法医学院,四川成都610041

出　　处：《华西药学杂志》2007年第1期9-12,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究枸杞籽油抗动脉粥样硬化的功效及其可能的作用机制。方法高脂膳食制备家兔动脉粥样硬化实验模型,观测其血浆中TC、TG、LDL-C、HDL-C、ApoA、ApoB的变化;56 d后,测定血清SOD、GsH-Px、T-AOC活性和MDA的含量;石蜡切片进行病理学检测;免疫印迹检测PKC、MMP-2、MMP-9在血管中的表达。结果枸杞籽油可增加实验家兔血浆中HDL-C、ApoA的含量,降低血浆中TC、TG、LDL-C、ApoB的含量,增强血清中SOD、GSH-PX、T-AOC的活性,降低血清中MDA的含量;降低PKC、MMP-2、MMP-9在血管中的表达。结论枸杞籽油具有显著的抗动脉粥样硬化效应。其机制可能是通过PKC途径,减少MMPs的分泌、抑制SMC的增生、分泌胶原、降血脂和抗氧化等。OBJECTIVE To study the anti - atherosclerosis potency of Lycium seed oil and its possible mechanism. METHODS The rabbit atherosclerosis model was established by high fat diet, and the TC, TG, LDL - C, HDL - C, ApoA, ApoB levels in plasma were examined dynamically. The SOD, GSH -PX,T -AOC activities and the MDA levels in serum were monitored after 8 week＇s high fat diet. Aorta samples were observed for atherosclerotic extent, and the expression of PKC and MMPs were measured by immunohisto- chemistry. RESULTS Contents of HDL - C and APOA obviously increased in plasma of low and high dosage Lycium seed oil groups, while TC ,TG, LDL- C and ApoB levels significantly decreased compared with the control group. The SOD, GSH -PX, T -AOC activi- ties up - regulated while the MDA levels decreased in Lycium seed oil groups compared with the control group. The expression of PKC, MMP - 2, MMP - 9 were significantly lower than those in the control group. CONCLUSION Lycium seed oil has potent anti - athero- sclerosis effects, its anti - atherosclerosis potency is similar with the lovastatin. The possible mechanism involves the decreasing of plasma lipids, anti - peroxidation and inhibiting the expression of PKC, MMP - 2, MMP - 9.

关 键 词：枸杞籽油 动脉粥样硬化 蛋白激酶C 基质金属蛋白酶 

分 类 号：R965[医药卫生—药理学]