尿素酶三甲基壳聚糖亚微球的制备及其相关性质的初步研究  

Preparation and characterization of urease N-trimethyl chitosan chloride submicrospheres

作　　者：陈浮[1] 黄园[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2007年第1期12-14,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省科技攻关项目(04SG022-009-5)

摘　　要：目的制备尿素酶三甲基壳聚糖亚微球,并对其相关性质进行初步研究。方法采用离子交联法制备尿素酶三甲基壳聚糖亚微球,激光纳米粒度仪测量粒度分布、多分散性和Zeta电位,透射电镜观察粒子的形态,比色法测定酶活性以间接测定包封率,并考察主要影响因素。结果所制备的尿素酶三甲基壳聚糖亚微球形态规则,粒径分布均匀,平均粒径为303.0nm,多分散系数(PD I)=0.175,Zeta电位为18.1 mV;包封率为89.69%。结论采用离子交联法可制备粒度分布均匀、形态规则、具有较高包封率的尿素酶三甲基壳聚糖亚微球。OBJECTIVE To prepare urease N - trimethyl chitosan chloride submicrospheres and study its characteristics. METHODS Urease N- trimethyl chitosan chloride submicrospheres were prepared by ionic crosslinking. Particle size, polydispersity index and zeta potential were determined by nanoparticle size analyzer. Morphology of the submicrospheres was examined by transmission electron microscopy. Urease activity was analyzed by eolorimetry in order to determine the entrapment efficiency indirectly. And the main factors that influenced the particle size and entrapment efficiency were investigated. RESULTS The morphology of urease N - trimethyl chitosan chloride submicrospheres was spherical and well distributed. The particle size was 303.0 nm with PDI of 0. 175 and zeta potential of 18.1 mV. The entrapment efficiency was 89.69%. CONCLUSION Spherical and well distributed urease N - trimethyl chitosan chloride submicrospheres with a high entrapmnet efficiency can be prepared by ionic crosslinking with tripolyphoph.

关 键 词：尿素酶 三甲基壳聚糖 亚微球 粒径 包封率 

分 类 号：R945[医药卫生—微生物与生化药学]