复方银黄微型灌肠剂对发热大鼠下丘脑中COX-1、COX-2 mRNA表达的影响  

Effect of Compound Yinhuang micro-enema on mRNA expression of cyclooxygenase-1 and cyclooxygenase-2 in hypothalamus of fever rats

作　　者：刘华钢[1] 阳洁[1] 杨斌[1] 赖茂祥[2] 蔡文娥[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西中医药研究所,广西南宁530022

出　　处：《华西药学杂志》2007年第1期14-16,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30260124)

摘　　要：目的研究复方银黄微型灌肠剂(YHCE)对发热大鼠下丘脑中环氧化酶-1(COX-1)、环氧化酶-2(COX-2)mRNA表达的影响,探讨其作用机制。方法用酵母混悬液复制发热大鼠模型,采用实时荧光定量聚合酶链反应检测大鼠下丘脑中COX-1、COX-2的mRNA表达的变化。结果YHCE能明显抑制发热大鼠的体温上升,抑制下丘脑中COX-2的mRNA表达,而对下丘脑中COX-1的mRNA无明显影响。结论YHCE有明显的解热作用,其机制可能与抑制发热大鼠下丘脑中COX-2的mRNA表达有关。OBJECTIVE To study the effect of the Compound Yinhuang micro - enema（YHCE） on mRNA expression of cyclooxygenase - 1 （ COX - 1 ） and eyclooxygenase - 2 （ COX - 2 ） in hypothalamus of fever rats and its mechanisms. METHODS The animal model was induced by injecting suspension of yeast hypodermically. The mRNA expression changes of COX - 1 and COX - 2 in hypothalamus of rats were detected by real time fluorescence quantitative polymerase chain reaction. RESULTS YHCE significantly inhibited the temperature rise and the mRNA expression of COX - 2. But it had no obvious effect on the mRNA expression of COX - 1. CONCLUSION YHCE has obvious antipyretic effect and its mechanism may be related to its inhibition of the mRNA expression of COX- 2 in hypothalamus of fever rats.

关 键 词：复方银黄微型灌肠剂 解热 环氧化酶-1 环氧化酶-2 实时荧光定量聚合酶链反应 

分 类 号：R285[医药卫生—中药学]