指纹图谱测定注射用血塞通、血栓通注射液及注射用血脉通的化学成分  

Determination of the effecient elements of Xuesaitong for injection,Xueshuantong injection and Xuemaitong for injection by fingerprint

作　　者：傅萍[1] 刘仲义[1] 

机构地区：[1]四川省食品药品检验所,四川成都610036

出　　处：《华西药学杂志》2007年第1期76-78,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究注射用血塞通、血栓通注射液及注射用血脉通的化学成分,为注射用血脉通立项提供实验依椐。方法用symetry shield C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-水二元梯度洗脱;流速1.0 ml.min-1;分别以ELSD和UVD为检测器,ELSD测定参数为漂移管温度115℃;气体(氮气)流量1.5 L.min-1,检测波长210 nm。在此色谱条件下,将3种药品的主要组分分别展开,表达成色谱指纹图谱,并对主组分进行结构识别,归属跟踪,对各自的有效成分进行了分析和比较。结果注射用血塞通、血栓通注射液均为三七的总皂苷,有效成分基本相似,仅存在组成比例波动,其主要差别是剂型不同;注射用血脉通的主组分为三七三醇皂苷,与前两制剂的物质基础存在较大差异。结论通过指纹图谱可以对三七及其制剂的有效成分进行测定。OBJECTIVE To analyze the effecient elements of Xuesaitong for injection, Xueshuantong injection and Xuemaitong for injection by fingerprint. METHODS A primary analysis was carried out on symetry shield C18 column （250 mm×4.6 mm,5 μm） , with acetonitrile and water as mobil phase （ griadient flow ） and flow rate of 1.0 ml· min^ - 1. ELSD and UVD were used as detectors. The temperature of drift tube was 115℃ with N2 flow rate of was 1.5 L·min^-1 for ELSD, while detection wavelength was 210 nm for UVD. CONCLUSION The difference lies in the effecient elements of Xuesaitong for injection, Xueshuantong injection and Xuemaitong for injection. RESULTS The fingerprint is suitable for the determination of notoginseng and its injections.

关 键 词：高效液相色谱法 蒸发光散射光检测法 指纹图谱 注射用血塞通 血栓通注射液 注射用血脉通 三七总皂苷 三七三醇皂苷 

分 类 号：R917[医药卫生—药物分析学]